摘要： TOXOPLASMA GONDII,AN OBLIGATE INTRACELLULAR PARASITE,CAN INFECT ALMOST ALL MAMMALIAN SPECIES AN... TOXOPLASMA GONDII,AN OBLIGATE INTRACELLULAR PARASITE,CAN INFECT ALMOST ALL MAMMALIAN SPECIES AND MAY LEAD TO TOXOPLASMOSIS IN THESE HOSTS.IN THE PRESENT STUDY,WE EXAMINED SEQUENCE VARIATION IN RHOPTRY PROTEIN 38 (ROP38) GENES AMONG T.GONDII ISOLATES FROM DIFFERENT HOSTS AND GEOGRAPHICAL REGIONS.THE COMPLETE ROP38 GENE FROM 13 T.GONDII ISOLATES WERE AMPLIFIED AND SEQUENCED.THE RESULTS OF SEQUENCE ALIGNMENTS SHOWED THAT THE LENGTHS OF THE ENTIRE ROP38 GENE RANGED FROM 2646 BP TO 2650 BP,AND THE SEQUENCE VARIATION WAS 0:1.1% AMONG THE 13 T.GONDII ISOLATES,INDICATING A LOW SEQUENCE VARIATION IN ROP38 GENE.PHYLOGENETIC ANALYSIS OF ROP38 SEQUENCES USING BAYESIAN INFERENCE (BI) SHOWED THAT THE CLUSTERING OF THE 13 T.GONDII ISOLATES WERE NOT CONSISTENT WITH THEIR RESPECTIVE GENOTYPES,INDICATING THAT THE ROP38 GENE IS NOT A SUITABLE GENETIC MARKER FOR POPULATION GENETIC STUDIES OF DIFFERENT T.GONDII GENOTYPES FROM DIFFERENT HOSTS AND GEOGRAPHICAL LOCATIONS,BUT MAY REPRESENT AN POTENTIAL VACCINE CANDIDATE AGAINST TOXOPLASMOSIS,WORTH FURTHER STUDIES.显示全部
