中文会议： 中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会论文集

会议日期： 2013-11-01

会议地点： 郑州

主办单位： 中国畜牧兽医学会

作　　者： ; ; ; ; ; ; ;

机构地区： 安徽农业大学动物科技学院

出　　处： 《中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会》

摘　　要： 　　TOXOPLASMA GONDII,AN OBLIGATE INTRACELLULAR PARASITE,CAN INFECT ALMOST ALL MAMMALIAN SPECIES AND MAY LEAD TO TOXOPLASMOSIS IN THESE HOSTS.IN THE PRESENT STUDY,WE EXAMINED SEQUENCE VARIATION IN RHOPTRY PROTEIN 38 (ROP38) GENES AMONG T.GONDII ISOLATES FROM DIFFERENT HOSTS AND GEOGRAPHICAL REGIONS.THE COMPLETE ROP38 GENE FROM 13 T.GONDII ISOLATES WERE AMPLIFIED AND SEQUENCED.THE RESULTS OF SEQUENCE ALIGNMENTS SHOWED THAT THE LENGTHS OF THE ENTIRE ROP38 GENE RANGED FROM 2646 BP TO 2650 BP,AND THE SEQUENCE VARIATION WAS 0:1.1% AMONG THE 13 T.GONDII ISOLATES,INDICATING A LOW SEQUENCE VARIATION IN ROP38 GENE.PHYLOGENETIC ANALYSIS OF ROP38 SEQUENCES USING BAYESIAN INFERENCE (BI) SHOWED THAT THE CLUSTERING OF THE 13 T.GONDII ISOLATES WERE NOT CONSISTENT WITH THEIR RESPECTIVE GENOTYPES,INDICATING THAT THE ROP38 GENE IS NOT A SUITABLE GENETIC MARKER FOR POPULATION GENETIC STUDIES OF DIFFERENT T.GONDII GENOTYPES FROM DIFFERENT HOSTS AND GEOGRAPHICAL LOCATIONS,BUT MAY REPRESENT AN POTENTIAL VACCINE CANDIDATE AGAINST TOXOPLASMOSIS,WORTH FURTHER STUDIES.

分 类 号： [ZZ]