摘要： GERMIN-LIKE PROTEINS (GLPS) PLAY DIVERSIFIED ROLES IN PLANT DEVELOPMENT AND DEFENSE RESPONSE.HE... GERMIN-LIKE PROTEINS (GLPS) PLAY DIVERSIFIED ROLES IN PLANT DEVELOPMENT AND DEFENSE RESPONSE.HERE, WE IDENTIFIED 36 EXPRESSED SEQUENCE TAGS (ESTS) ENCODING GLPS FROM PEANUT (ARACHIS HYPOGAEA L.).AFTER ASSEMBLY, THESE ESTS WERE INTEGRATED INTO EIGHT UNIGENES NAMED AHGLP1 TO AHGLP8, OF WHICH, THREE (AHGLP1-3) WERE COMPRISED 14, TEN, AND SEVEN EST CLONES, RESPECTIVELY, WHEREAS THE REMAINING ONES WERE ASSOCIATED WITH ONE SINGLE CLONE.THE LENGTH OF THE DEDUCED AMINO ACID (AA) RESIDUES RANGED FROM 208 TO 223 AAS EXCEPT FOR AHGLP6 AND AHGLP8, WHICH WERE INCOMPLETE AT THE CARBOXYL TERMINUS.ALL OF THE AHGLPS CONTAINED A POSSIBLE N-TERMINAL SIGNAL PEPTIDE THAT WAS 17 TO 24 RESIDUES IN LENGTH EXCLUDING AHGLP7, WHERE THERE IS LIKELY A NON-CLEAVABLE AMINOTERMINUS.PHYLOGENETIC ANALYSIS SHOWED THAT THESE AHGLPS WERE CLASSIFIED INTO THREE SUBFAMILIES.SOUTHERN BLOT ANALYSIS INDICATED THAT AHGLP1 AND AHGLP2 LIKELY HAVE MULTIPLE COPIES IN THE PEANUT GENOME.THE RECOMBINANT MATURE AHGLP1 AND AHGLP2 PROTEINS WERE SUCCESSFULLY EXPRESSED IN ESCHERICHIA COLI.THE PURIFIED AHGLP2 HAS SUPEROXIDC DISMUTASE (SOD) ACTIVITY IN ENZYMATIC ASSAY, BUT NOT OXALATE OXIDASE ACTIVITY.THE SOD ACTIVITY OF AHGLP2 WAS STABLE UP TO 70℃ AND RESISTANT TO HYDROGEN PEROXIDE, SUGGESTING THAT AHGLP2 MIGHT BE A MANGANESECONTAINING SOD.FURTHERMORE, AHGLP2 COULD CONFER E.COLI RESISTANCE TO OXIDATIVE DAMAGE CAUSED BY PARAQUAT, SUGGESTING THAT THE AHGLP2 LIKELY PROTECTS PEANUT PLANTS FROM REACTIVE OXYGEN METABOLITES.THUS, INFORMATION PROVIDED IN THIS STUDY INDICATES THE DIVERSE NATURE OF THE PEANUT GLP FAMILY AND SUGGESTS THAT SOME OF AHGLPS MIGHT BE INVOLVED IN PLANT DEFENSE RESPONSE.显示全部
