作　　者： ; (李珂）;

机构地区： 肇庆医学高等专科学校

出　　处： 《食品与机械》 2018年第7期158-163,共6页

摘　　要： 以广东德庆地区的巴戟天为原料,对巴戟天多糖提取工艺进行响应面优化分析,并对提取所得巴戟天多糖的抗氧化性及抗疲劳作用进行评价。结果表明,最佳提取条件为提取时间2h,提取次数3次,料液比1:11(g/mL),巴戟天多糖得率为6.71%,与理论预测值仅相差0.11%;体外抗氧化研究表明,在该条件下得到的巴戟天多糖具有良好的O_2^-·和DPPH·清除能力。表明优化所得工艺条件稳定可行,且所得多糖具有良好抗氧化能力。NIH小鼠试验结果显示,巴戟天多糖能降低体内的尿素氮和乳酸,升高肝糖元含量,呈现出一定的抗疲劳效用。 Morinda officinalis polysaccharide is an important bioactive substance,in terms of its anti-depression,immune enhancement,anti-viral and anti-tumor effects,and polysaccharide holds great potential in function food and drug development.In this study,the Box-Behnken design principle and one-factor-at-a-time experiments were employed to optimize the extraction parameters of polysaccharide from Morinda officinalis How in Deqing area,Guangdong province.The in vitro antioxidant activities of extracted polysaccharides were also investigated.The results showed that the optimal extraction conditions were as follows：extraction time was2 h,extraction times was 3,and solid-liquid ratio was 1：11（g/mL）.Under the control of these conditions,the polysaccharide yield of Morinda officinalis was 6.71%,which was only less 0.11% than the theoretically predicted value.The obtained Morinda officinalis polysaccharides have a potent O_2^-· and DPPH· scavenging capacity,indicating that the optimized process conditions were stable and feasible.The blood urea nitrogen（BUN）and arterial blood lactate concentration（LACT）were decreased while the hepatic glycogenosis（HG）was increased after treated with different concentrations of polysaccharides of Morinda officinalis How in NIH mice,which indicates that the polysaccharides of Morinda officinalis How has anti-fatigue in vivo.

关 键 词： 巴戟天 多糖 抗氧化 抗疲劳

领　　域： [医药卫生—中药学] [医药卫生—中医学]