作　　者： (张勇）; ; (林大都）; (张声源）; (谢华松）;

机构地区： 嘉应学院医学院

出　　处： 《食品工业科技》 2021年第14期49-54,共6页

摘　　要： 以福建观音座莲叶为材料,探究其乙醇提取物不同溶剂萃取部位中总黄酮、总酚的含量及抗氧化活性,并分析其相关性。采用Al(NO_(3))_(3)络合法和福林酚法分别测定提取物各萃取部位中总黄酮、总多酚的含量,以(DPPH、ABTS、普鲁士蓝)法和脂质过氧化抑制能力综合评价各部位抗氧化活性,采用Pearson法分析2种成分与抗氧化活性的相关性。结果表明,福建观音座莲叶乙酸乙酯部位中总黄酮含量(271.05±2.82 mg/g)与总酚含量(59.62±3.23 mg/g)均最高;各部位均具有较好抗氧化活性,其中乙酸乙酯部位抗氧化能力最强,呈明显量效关系。相关性分析显示,各部位抗氧化活性与其总黄酮、总多酚含量呈极显著正相关(P<0.01)。因此,福建观音座莲叶各部位均含有总酚、总黄酮成分和抗氧化活性,乙酸乙酯部位最佳,可能活性成分为酚类和黄酮类化合物。 In this study,the leaves of Angiopteris fokiensis Hieron were used as materials to explore the contents and antioxidant activities of total flavonoids and total phenols in different solvent extraction parts of its ethanol extracts,and their correlation was analyzed.The contents of total flavonoids and total polyphenols in each extraction part were determined by Al(NO_(3))_(3) complexation method and Folin-phenol method,respectively.The antioxidant activity of each part was evaluated by(DPPH,ABTS,Prussian blue)method and lipid peroxidation inhibition ability.Pearson method was used to analyze the correlation between the two components and antioxidant activity.The results showed that the contents of total flavonoids(271.05±2.82 mg/g)and total phenols(59.62±3.23 mg/g)in the ethyl acetate site of the leaves of Angiopteris fokiensis Hieron were the highest,and all parts had good antioxidant activity,among which the ethyl acetate site had the strongest antioxidant activity in a dose-dependent manner.Correlation analysis showed that the antioxidant activity of each part was significantly positively correlated with the contents of total flavonoids and total polyphenols(P<0.01).Therefore,all parts of the Angiopteris fokiensis Hieron had the contents of total flavonoids and total phenolsand and antioxidant effects,and the ethyl acetate part was the best.The active ingredient might be total flavonoids and total phenols.

关 键 词： 福建观音座莲叶 萃取部位 总黄酮 总酚 抗氧化活性

领　　域： [轻工技术与工程—食品科学] [轻工技术与工程—食品科学与工程]