作　　者： (牛晓君）; ; (林小艺）;

机构地区： 华南理工大学环境与能源学院

出　　处： 《环境工程学报》 2017年第5期2797-2803,共7页

摘　　要： 采用室内模拟实验,在具有3个格室的ABR中进行连续流实验,分别在有无四环素的条件下运行,定期采集反应器内各格室产生的气体和活性污泥样本,分析氢气甲烷产量的变化,并用Illumina Mi Seq高通量测序方法对活性污泥中微生物的组成情况进行基因分析,探讨四环素对污水厌氧消化系统微生物群落结构造成的影响。结果表明:四环素的添加能使氢气产量增加,四环素对产甲烷的抑制作用随着时间的推移增大;Illumina Mi Seq测序获得反应器内的序列为16个属,其中,密螺旋体属(Treponema)、脱硫弧菌属(Desulfovibrio)、互营杆菌属(Syntrophobacter)、W22对四环素具有一定耐药性,反应器内部分微生物在四环素长期污染下耐药性发生改变;添加四环素前后系统中物种组成具有较大差异,随着运行时间的推移,格室间物种相似性增大。 The influence of tetracycline（TC） on microbial community structure in anaerobic digestion system has been studied.A lab-scale anaerobic baffled reactor（ABR） with three compartments was employed to simulate this process.It was continuously fed with synthetic wastewater.Illumina Mi Seq high-throughput sequencing was used to analyze microbial components in activated sludge.The produce of H_2,CH_4 and the microbial components in activated sludge were analyzed periodically.Results showed that TC enhanced the produce of H_2 but inhibited the produce of CH_4,and this inhibitive effect increased as time processing.Sequence analysis showed 16 genera in the reactor,among which Treponema,Syntrophobacter,Desulfovibrio,W22 presented TC resistance,and the resistance performance of some genera changed after a long-term TC exposure.Besides,microbial community structure in the reactor showed a great shift after TC addition,and the similarity of microbial components among compartments increased with TC exposure.

关 键 词： 四环素 厌氧消化系统 气体产生 微生物群落结构 高通量测序

领　　域： [环境科学与工程—环境工程]