作　　者： (）;

机构地区： 广州医科大学附属肿瘤医院

出　　处： 《中国医师杂志》 2018年第11期1613-1616,共4页

摘　　要： 目的探讨活化的肿瘤相关巨噬细胞对乳腺癌T47D细胞侵袭和迁移的影响。方法使用佛波酯(PMA)体外诱导单核细胞THP-1,并用白细胞介素4(IL-4)诱导建立活化的肿瘤相关巨噬细胞模型。用未活化M0型和活化的M2型巨噬细胞培养液上清处理乳腺癌细胞T47D,细胞划痕实验和transwell实验检测T47D细胞迁移和侵袭能力。qRT-PCR和Western blot实验检测细胞上皮-间质转化(EMT)标志物的表达。结果成功建立未活化M0型巨噬细胞和M2型肿瘤相关巨噬细胞模型。采用巨噬细胞培养上清处理T47D细胞,结果发现与M0型巨噬细胞培养上清相比,M2型巨噬细胞上清显著增加T47D细胞的侵袭和迁移能力。qRT-PCR和Western blot提示M2型巨噬细胞上清可显著下调钙黏附蛋白E(E-cadherin),上调波形蛋白(vimentin)、钙黏附蛋白N(N-cadherin)和β-连环蛋白(β-catenin)的表达。结论活化的M2型肿瘤相关巨噬细胞可促进乳腺癌细胞T47D发生EMT表型改变,进而增加T47D细胞侵袭和迁移能力。 Objective To explore the effect of tumor-associated macrophage (TAM) on invasion and migration of breast cancer cells T47D.Methods Briefly,phorbol 12-myristate 13-acetate (PMA) was employed to treat mononuclear cells THP-1.And then interleukin 4 (IL-4) was applied to stimulate mentioned cells to establish activated TAM.Furthermore,the supernatant of M0 and M2 was used to culture T47D cells,respectively.Then,the wound healing experiment and transwell assay were carried out to investigate cells invasion and migration.Finally,epithelial-mesenchymal transition (EMT) biomarkers in T47D cells treated with M0 or M2 were determined by quantitative real time polymerase chain reaction (qRT-PCR) and western blot assays.Results Activated TAM model was successfully established by PMA following with IL-4.Comparing with M0,M2 evidently accelerated invasion and migration in T47D cells.Meanwhile,M2 upregulated N-cadherin,vimentin and β-catenin,downregulated E-cadherin at mRNA and protein expression levels.Conclusions M2 promotes invasion and migration of T47D cells via EMT.

关 键 词： 巨噬细胞 乳腺肿瘤 细胞迁移分析 体外研究

领　　域： []