作　　者： (）;

机构地区： 广西中医药大学药学院

出　　处： 《广西师范大学学报（自然科学版）》 2020年第3期85-91,共7页

摘　　要： 建立同时测定广地龙中5种核苷类成分含量的HPLC法。采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(5∶95,体积比)等度洗脱,流速1.0 mL/min,检测波长254 nm,柱温30℃,测定了广地龙药材中5种核苷类成分的含量。结果表明:尿嘧啶、次黄嘌呤、尿苷、肌苷、鸟苷分别在14.84~111.30 ng(r=0.999 9)、126.00~945.00 ng(r=0.999 8)、63.60~477.00 ng(r=0.999 9)、612.00~4 590.00 ng(r=0.999 8)、41.60~312.00 ng(r=0.999 9)线性关系良好,平均加样回收率(n=9)分别为99.27%、99.14%、98.59%、98.69%、99.91%,RSD(n=9)分别为0.95%、0.86%、1.01%、0.86%、1.91%;20批广地龙中尿嘧啶、次黄嘌呤、尿苷、肌苷、鸟苷含量分别为0.051 0~0.111 0、0.243 1~0.943 6、0.171 9~0.508 0、1.559 6~3.861 1、0.165 5~0.250 1 mg/g,5种总核苷含量为3.309 9~4.565 9 mg/g。该方法简便、可靠、重复性好、精密度高,可用于同时测定广地龙中5种核苷类成分的含量。 To establish an HPLC method for the quantitative of five nucleoside components in Pheretima aspergillum(E. Perrier).The mobile phase was eluted with Diamonsil C18(250 mm× 4.6 mm, 5 μm) by using methanol-water solution(5∶95) such as mobile phase with a flow rate of 1.0 mL/min and a detection wavelength of 254 nm. The column temperature was 30 ℃. The contents of 5 nucleoside components in P. aspergillum(E. Perrier) were determined. Uracil, hypoxanthine, uridine, inosine and guanosine showed a good linear relationship in the range of 14.84~111.30 ng(r=0.999 9), 126.00~945.00 ng(r=0.999 8), 63.60~477.00 ng(r=0.999 9), 612.00~4 590.00 ng(r=0.999 8) and 41.60~312.00 ng(r=0.999 9), with average recovery(n=9) of 99.27%, 99.14%, 98.59%, 98.69% and 99.91%, respectively. The RSD(n=9)was 0.95%, 0.86%, 1.01%, 0.86% and 1.91%, respectively. The contents of uracil, hypoxanthine, uridine, inosine and guanosine in 20 batches were 0.051 0~0.111 0, 0.243 1~0.943 6, 0.171 9~0.508 0, 1.559 6~3.861 1 and 0.165 5~0.250 1 mg/g, respectively. The content of 5 nucleosides was 3.309 9~4.565 9 mg/g. The established method was simple, reliable, reproducible and accurate, and can be used for the simultaneous determination of 5 nucleosides in P. aspergillum(E. Perrier).

关 键 词： 广地龙 核苷类 含量测定

领　　域： []