作　　者： ; ; ; ;

机构地区： 暨南大学

出　　处： 《中国现代中药》 2020年第1期98-102,共5页

摘　　要： 目的:比较茵陈饮片与茵陈标准汤剂的质量。方法:按照标准汤剂制备要求,制备12批茵陈饮片标准汤剂,测定绿原酸含量,计算其转移率,测定汤剂的出膏率,并建立茵陈饮片与标准汤剂的HPLC指纹图谱,以0.1%甲酸水(A)-乙腈(B)为流动相,梯度洗脱,柱温为30℃,流速为1 mL·min^-1,检测波长为330 nm。结果:12批茵陈饮片标准汤剂的绿原酸转移率为31.0%~62.4%,出膏率21.2%~30.1%,茵陈饮片与标准汤剂指纹图谱相似度均>0.96,指认了7个共有峰,分别为新绿原酸、绿原酸、隐绿原酸、咖啡酸、1,3-二咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸。结论:该研究中茵陈饮片标准汤剂制备方法规范,指纹图谱相似度高,其方法精密度、稳定性和重复性良好,可为茵陈饮片标准汤剂质量控制提供参考。 Objective:Compare the quality of Artemisia capillaris and standard decoction.Methods:12 batches of Artemisia capillaris standard decoctions were prepared following the preparation conditions of the TCM standard decoction.The transfer rate of chlorogenic acid and extract rate were calculated.HPLC fingerprints of Artemisia capillaris and its standard decoction were established under a flow rate of 1.0 mL·min^-1 and eluted with a mobile phase of acetonitrile(B)-0.1%formic acid acid solution(A)in a gradient mode.The column temperature was set at 30℃and the detection wavelength was 330 nm.Results:the chlorogenic acid transfer rate of standard decoctions was at a range of 31.0%to 62.4%,the extract rate was from 21.2%to 30.1%.The fingerprint similarities of Artemisia capillaris and its standard decoction were all greater than 0.96,and seven were identified,namely,neochlorogenic acid and chlorogenic acid.cryptochlorogenic acid,caffeic acid,1,3-dicaffeoylquinic acid,3,4-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid.Conclusion:In this study,the preparation method for Artemisia capillaris decoction was standard,with high similarity in fingerprint,and showing high precision,stability and repeatability in fingerprint analysis.Thus,this study can provide a reference for the quality control of Artemisia capillaris standard decoction.

关 键 词： 茵陈 标准汤剂 绿原酸 指纹图谱 质量评价

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