作　　者： ;

机构地区： 中山市人民医院

出　　处： 《国际呼吸杂志》 2020年第4期268-273,共6页

摘　　要： 目的探讨三重基序家族蛋白(TRIM72)在肺腺癌组织及外周血、胸腔积液中的表达。方法收集2017年8月至2018年2月广东医科大学附属医院确诊的肺腺癌患者80例,并以同时段20例健康体检者作为对照组。通过免疫组织化学方法进行TRIM72表达检测。采用TRIM72酶联免疫吸附试验试剂盒对外周血、胸腔积液进行检测,并分析临床数据。结果TRIM72主要表达于正常气道上皮细胞中,而在肺腺癌组织中几乎不表达。肺腺癌患者外周血TRIM72水平明显低于对照组(t=8.727,P<0.05),与癌胚抗原呈负相关性(r=-0.556,P<0.001)。癌性胸腔积液TRIM72水平明显低于炎性积液(t=10.09,P<0.05)。采用TRIM72、CEA、CA125等指标对肺腺癌诊断作受试者工作曲曲线下面积分别为0.827、0.746、0.651,cut-off值分别为110.629 ng/L、4.535μg/L、33.965 U/ml,TRIM72同时联合CEA及CA125曲线下面积可达0.934。结论TRIM72可能作为肺腺癌诊断的生物学标记物,与肺腺癌的发生、发展及预后相关。 Objective To explore the expressions of tripartite motif family protein 72(TRIM72)in peripheral blood,pleural effusion and tumor tissue in patients with lung adenocarcinoma.Methods There were 80 cases diagnosed with lung adenocarcinoma collected in the affiliated hospital of Guangdong Medical University from August 2017 to February 2018 and 20 healthy subjects in the same period were taken as control group.The expression of TRIM72 was detected by immunohistochemistry,and ELISA kits were used to detect the TRIM72 in peripheral blood and pleural effusion.Also,clinical data were analyzed.Results TRIM72 was mainly expressed in normal airway epithelial cells but hardly expressed in lung adenocarcinoma tissues.TRIM72 level of peripheral blood was significantly lower in the patients with lung adenocarcinoma compared to control group(t=8.727,P<0.05),and negatively correlated with CEA(r=-0.556,P<0.001).TRIM72 level in malignant pleural effusion is significantly lower than that in inflammatory effusion(t=10.09,P<0.05).TRIM72,CEA,CA125 were used to make receiver operating characteristics curve for the diagnosis of lung adenocarcinoma.The areas under the curve were 0.927,0.746,0.851,with a cut-off 110.629 ng/L,4.535μg/L,33.965 U/ml respectively.When TRIM72 combined with CEA and CA125,the area under the curve could reach 0.974.Conclusions Serum TRIM72 may be a potential biomarker for the diagnosis and the prognosis of lung adenocarcinoma.

关 键 词： 生物学标记 受试者工作特征曲线 三重基序家族蛋白 肺腺癌

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