作　　者： ; ;

机构地区： 江门市中心医院

出　　处： 《中外医学研究》 2020年第15期65-67,共3页

摘　　要： 目的:探讨间接免疫荧光法测定抗核抗体诊断鼻咽癌的可能性。方法:收集2018年6月-2019年10月江门市中心医院收治的129例鼻咽癌,77例健康体检标本作为对照组,通过间接免疫荧光法检测血清中抗核抗体。结果:抗核抗体阳性率分别为Ⅰ~Ⅱ期鼻咽癌组52.9%,Ⅲ~Ⅳ期鼻咽癌组62.1%,对照组18.2%,三组比较差异有统计学意义(χ^2=11.809,P=0.003);Ⅰ~Ⅱ期鼻咽癌组与Ⅲ~Ⅳ期鼻咽癌组分别与对照组比较,差异有统计学意义(χ^2=9.972、8.015,P=0.002、0.005);Ⅰ~Ⅱ期鼻咽癌组和Ⅲ~Ⅳ期鼻咽癌组比较,差异无统计学意义(χ^2=0.874,P=0.310)。抗核抗体核荧光核型比较,两组均以抗细胞核抗体为主,鼻咽癌组比对照组多了周期蛋白型、着丝点型和纺锤体型。抗核抗体荧光滴度比较,滴度1∶100,鼻咽癌组46.1%,对照组78.6%,差异有统计学意义(χ^2=5.003,P=0.025);滴度1∶320,鼻咽癌组38.2%,对照组21.4%,差异无统计学意义(χ^2=1.444,P=0.229);滴度≥1∶1000,鼻咽癌组15.8%,对照组无。结论:鼻咽癌患者血清的抗核抗体不同于健康者生理性自身抗体,抗核抗体对鼻咽癌的诊断提供了一定的依据。 Objective:To investigate the possibility of diagnosis of nasopharyngeal carcinoma by antinuclear antibody indirect immunofluorescence assay.Method:A total of 129 cases of nasopharyngeal carcinoma admitted to the Jiangmen Central Hospital from June 2018 to October 2019 were collected,and 77 healthy physical examination specimens were used as the control group.Determined antinuclear antibody by indirect immunofluorescence assay.Result:The positive rates of antinuclear antibodies were 52.9% in group Ⅰ-Ⅱ nasopharyngeal carcinoma,62.1% in group Ⅲ-Ⅳ nasopharyngeal carcinoma,and 18.2% in control group.The difference among the three groups was statistically significant(χ^2=11.809,P=0.003).The difference between theⅠ-Ⅱnasopharyngeal carcinoma group and the Ⅲ-Ⅳ nasopharyngealcarcinoma group and the control group was statistically significant(χ^2=9.972,8.015;P=0.002,0.005).There was no statistical difference between the Ⅰ-Ⅱ nasopharyngeal carcinoma group and the Ⅲ-Ⅳ nasopharyngeal carcinoma group(χ^2=0.874,P=0.310).The anti-nuclear antibody fluorescence titer was 1∶100,46.1% in the nasopharyngeal carcinoma group and 78.6%in the control group,the difference was statistically significant(χ^2=5.003,P=0.025).The titer was 1∶320,38.2% in the nasopharyngeal carcinoma group and 21.4% in the control group,the difference was not statistically significant(χ^2=1.444,P=0.229).The titer was≥1∶1000,15.8% in the nasopharyngeal carcinoma group,and 0 in the control group.Compared with the nuclear nucleus of antinuclear antibody,both groups were mainly anti-nuclear antibodies,and the nasopharyngeal carcinoma group had more cyclin,centromere and spindle type than the control group.Conclusion:The anti-nuclear antibodies in the serum of patients with nasopharyngeal carcinoma are different from the healthy self-antibody antibodies,and anti-nuclear antibodies provide a basis for the diagnosis of nasopharyngeal carcinoma.

关 键 词： 抗核抗体 间接免疫荧光法 鼻咽癌

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