作　　者： ; ; ; ; ; ;

机构地区： 北京大学深圳医院

出　　处： 《中国组织工程研究》 2020年第11期1683-1688,共6页

摘　　要： 背景:近年的研究表明,中医推拿能够减轻甚至逆转肌肉纤维化的过程,因此推测推拿也能够改善臀肌挛缩症的病理变化。目的:初步探讨推拿治疗对于臀肌挛缩症的影响及其机制。方法:用20只幼兔为实验对象,将其分为实验组和对照组,两组均给予青霉素钠+苯甲醇连续注射8周建立臀肌挛缩模型。实验组接受手法按摩4周,对照组则不作特殊处理。观察两组兔臀肌组织标本苏木精-伊红染色、Masson染色及天狼星红染色下细胞形态和结构变化,并采用RT-PCR、Western blot、免疫组织化学等方法分析纤维挛缩带中转化生长因子β1的表达及其作用机制。实验方案经北京大学深圳医院伦理委员会批准。结果与结论:①兔臀肌组织病理学观察显示,实验组标本较对照组的纤维增生程度明显减轻(P<0.05);②RT-PCR、Western blot检测及免疫组织化学染色显示,未经推拿的对照组中Ⅰ型胶原合成增加、转化生长因子β1的表达显著上调(P<0.05);③结果说明,推拿治疗可以有效缓解臀肌的纤维化进程,有望为臀肌挛缩症的机制研究和治疗提供新的思路。 BACKGROUND:Recent studies have shown that TCM massage can alleviate or even reverse the progression of muscle fibrosis,so it is speculated that massage can also improve the pathological changes of gluteal muscle contracture.OBJECTIVE:To evaluate the role of massage on gluteal muscle contracture and the relevant mechanism.METHODS:Twenty young rabbits were divided into an experimental group and a control group,with penicillin and benzyl alcohol injection for 8 weeks respectively.Besides,the experimental group received massage therapies for 4 weeks,whereas the control group had no specific intervention.Cell morphology and structure were observed using hematoxylin and eosin staining,Masson staining and Sirius red staining.Expression of transforming growth factor-β1 in the rabbit model of gluteal muscle contraction was measured using immunohistochemistry,reverse transcription PCR and western blot assays.The study protocol was approved by the Ethics Committee of Peking University Shenzhen Hospital in China.RESULTS AND CONCLUSION:The experimental group had significantly less hyperplasia of fiber tissue compared with the control group(P<0.05).The expression levels of collagen type I and transforming growth factor-β1 were significantly increased in the control group compared with the experimental group,as determined by reverse transcription PCR,western blot assay and immunohistochemical staining(P<0.05).Therefore,massage therapy can alleviate the progression of gluteal fibrosis,which is expected to provide a new idea for the research and treatment of gluteal muscle contracture.

关 键 词： 按摩 臀肌挛缩症 转化生长因子 幼兔

领　　域： []