作　　者： ; ; ; ; ;

机构地区： 中山大学附属第三医院

出　　处： 《皮肤性病诊疗学杂志》 2020年第2期72-78,共7页

摘　　要： 目的:探讨球形马拉色菌过增殖小鼠特应性皮炎(AD)模型的建立方法。方法:将18只BALB/c小鼠随机分为6组:对照组、AD组、NS/M组、AD+NS/M组、Oil/M组和AD+Oil/M组,每组3只。通过耳皮肤涂布MC903溶液诱导小鼠特应性皮炎模型后,再分别使用橄榄油和生理盐水作为基质配置球形马拉色菌混悬液涂抹小鼠耳皮肤,以构建真菌过增殖模型,并通过观察其临床表现、皮损评分、真菌学和组织病理学变化,扫描/透射电镜观察毛发结果等评估。结果:与AD组相比:AD+Oil/M组小鼠耳部湿疹样皮炎加重,耳周毛发有明显脱落,皮损评分升高(q=5.95,P<0.01);组织病理学结果显示AD+Oil/M组表皮明显增厚,真皮浅层致密的炎症细胞浸润,伴部分毛囊受损,毛干退化;PAS染色和真菌荧光染色可见角质层内大量球形马拉色菌;扫描/透射电镜下见毛干表面及毛干内均有大量球形马拉色菌。NS/M组和AD+NS/M组小鼠耳组织PAS染色和真菌荧光染色仅见散在数个马拉色菌。结论:应用橄榄油混悬球形马拉色菌液涂布皮肤可成功建立马拉色菌过增殖小鼠特应性皮炎模型。 Objective:To establish a mouse model of atopic dermatitis(AD)with overgrowth Malassezia globosa.Methods:Eighteen BALB/c mice were randomly divided into the control group,AD group,NS/M group,AD+NS/M group,Oil/M group and AD+Oil/M group,3 mice per group.AD model of BALB/c mice were established by topical administration of MC903.Fungi overgrowth experimental models were attempted to establish using suspension of M.globosa with olive oil and normal saline as vehicle,respectively.Clinical manifestations and lesion scoring as well as mycological and histopathological changes of each group were evaluated.Scanning and transmission electron microscopy were also used to observe the changes on hair follicle and hair shaft.Results:Compared with AD group,more severe AD-like lesion,obvious hair loss andincreasedlesion scoring(q=5.95,P<0.01)were observed in AD+Oil/M group.Histopathological results showed that the epidermis thickness of AD+Oil/M group was significantly increased,the dermis was infiltrated by more dense inflammatory cells,and M.globosa colonization lead to damaged hair folliclesand the degeneratedhair shafts.The results of PAS and fungal fluorescence staining showed a mass of M.globosain the stratum corneum of ear tissues.Overgrowth of M.globosa inside and outside the hair shaft were proved by scanning and transmission electron microscopy.In NS/M group and AD+NS/M group,only a few M.globose yeast cells were observed in the ear tissue using PAS and fungal fluorescence staining,respectively.Conclusion:A mouse model of AD with overgrowth M.globosa could be established treating with suspension of M.globosa using olive oil but not normal saline.

关 键 词： 球形马拉色菌 特应性皮炎 小鼠模型 橄榄油

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