作　　者： (）;

机构地区： 深圳市光明新区人民医院泌尿外科广东深圳518106

出　　处： 《分子影像学杂志》 2018年第1期68-71,共4页

摘　　要： 目的探讨miR-429在膀胱癌中的表达及作用机制。方法选取广东省内三家医院2014年1月~2016年12月期间收集的膀胱癌标本120例,同时选取距离肿瘤边缘大于2 cm的癌旁组织作为对照。SYBR green实时荧光定量PCR法检测miR-429在膀胱癌、癌旁组织中的相对表达量,并分析miR-429与临床相关参数的关系,同时观察miR-429过表达或者敲除时T24细胞(膀胱癌细胞系)增殖和凋亡情况。结果膀胱癌组织miR-429的相对表达水平高于正常膀胱组织(P<0.05);miR-429的表达水平与膀胱癌的肿瘤大小、有无淋巴结转移,及TNM分期有关(P<0.05);肿瘤直径大于5 cm组miR-429的表达水平高于肿瘤直径小于5 cm组;有淋巴结转移的膀胱癌组miR-429的表达水平高于无淋巴结转移的膀胱癌组;Ⅲ+Ⅳ期膀胱癌组miR-429的表达水平高于Ⅰ+Ⅱ期膀胱癌(P<0.05)。miR-429的表达水平与膀胱癌患者的年龄、性别无关(P>0.05)。miR-429过表达时,膀胱癌细胞(T24细胞系)增殖增加,凋亡减少;而miR-429敲除时,膀胱癌细胞(T24细胞系)增殖减少,凋亡增加。结论 miR-429在膀胱癌的发生发展中起着重要作用,其表达水平对膀胱癌的严重程度及预后情况具有一定的参考价值。 Objective To explore the expression and mechanism of miR-429 in bladder cancer. Methods A total of 120 cases with bladder cancer were collected from our hospital in 2015.1-2016.12. The adjacent tissues with the edge 〉2 cm were selected as the control. All specimens were confirmed by pathological diagnosis without preoperative radiotherapy and chemotherapy. The relative expression of miR-429 in bladder cancer and adjacent tissues were analyzed by SYBR green real-time quantitative PCR. The relative expression of miR-429 in bladder cancer carcinoma was explored and the relationship between miR-429 and clinical features was analyzed. Results The relative expression level of miR-429 in bladder cancer tissues was significantly higher than that in normal bladder tissues （P〈0.05）.The expression level of miR-429 was associated with tumor size, lymph node metastasis and TNM staging of bladder cancer （P〈0.05）. The expression level of miR-429 in tumor tissue with diameter greater than 5 cm was higher than that in tumor group with diameter less than 5 cm. The expression level of miR-429 in bladder transition group was higher than that in bladder transition group without lymph node metastasis.The expression level of miR-429 was significantly higher than that of stage Ⅰ ＋ Ⅱ bladder cancer （P〈0.05）. The expression level of miR-429 was not related to the age and sex of bladder cancer patients （P〉0.05）. miR-429 overexpression, bladder cancer cells （T24 cell line） proliferation increased and apoptosis reduced.miR-429 knockout, bladder cancer cells （T24 cell line） proliferation reduced.The apoptosis increased. Conclusion miR-429 is important in the development and progression of bladder cancer. Expression of miR-429 can provide references for the severity and prognosis of bladder cancer.

关 键 词： 膀胱癌

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