作　　者： (）;

机构地区： 广东药科大学基础学院广东省生物活性药物研究重点实验室

出　　处： 《广东药科大学学报》 2017年第6期768-772,共5页

摘　　要： 目的观察原花青素对H_2O_2诱导SH-SY5Y细胞氧化损伤的保护作用,并初步探讨其保护机制。方法 MTT法筛选合适的原花青素作用浓度,用H_2O_2(400μmol/L)建立SH-SY5Y细胞氧化损伤模型,分别用质量浓度为6.25、12.5、25μg/mL原花青素预处理各组细胞,倒置显微镜观察SH-SY5Y细胞形态学的变化;MTT法检测细胞存活率;Western-blot检测各组细胞HSPA1L蛋白表达水平的变化。结果与模型组比较,各浓度原花青素组的细胞形态明显改善,细胞存活率明显升高,呈浓度依赖性;与模型组比较,12.5、25μg/mL原花青素组HSPA1L表达水平逐渐降低,其中25μg/mL组HSPA1L表达水平显著下降(P<0.01)。结论原花青素可缓解H_2O_2诱导的SH-SY5Y细胞氧化损伤,其机制可能与调节H_2O_2诱导的HSPA1L蛋白表达有关。 Objective To study the protection and mechanism of proanthocyanidins on oxidative damage in SH-SY5Y cells induced by H_2O_2. Methods MTT method was used to select the useful concentration of proanthocyanidins. The model of oxidative damage on SH-SY5Y cells was established with H_2O_2（ 400μmol/L） treatment. The cells were pretreated with 6. 25,12. 5 and 25 μg/mL proanthocyanidins. The changes of SH-SY5Y cell morphology were observed by inverted microscope. MTT method was used to detect the survival rate of cells, and the expression of HSPA1L protein was detected by western blot.Results Compared with the model group,the cell morphology and survival rate were significantly improved after proanthocyanidins treatment in a dosage-dependent manner. Meantime, the level of HSPA1L was significantly decreased in 25 μg/mL proanthocyanidins group. Conclusion Proanthocyanidins can protect SH-SY5Y cells from oxidative damage induced by H_2O_2, and its mechanism may be related to modulate HSPA1L expression.

关 键 词： 原花青素 细胞 蛋白

领　　域： []