作　　者： ; ; ; ;

机构地区： 广东海洋大学水产学院

出　　处： 《中国水产科学》 2020年第5期494-503,共10页

摘　　要： 为了探究小分子热休克蛋白基因HSPB1、HSPB7和HSPB11在松江鲈(Trachidermus fasciatus)应对低盐胁迫过程中的调节作用,本研究基于前期转录组数据,获取3个目标基因的序列信息并进行了系统进化分析,利用实时荧光定量PCR技术检测了3个基因在两种低盐胁迫处理下不同时间点(0 h、12 h、24 h和48 h)在鳃、肠、肾和肝组织中的表达水平。系统进化分析结果表明, HSPB1、HSPB7和HSPB11基因分别聚类形成独立分支;在各基因分支中,松江鲈与已报道的鲈形目、鲤形目和鳉形目等鱼种共同聚为硬骨鱼类分支。在两种低盐胁迫处理下, 3个基因在鳃组织中的表达量均在12h显著升高,而在肠、肾和肝组织中的表达量则呈现不同的变化趋势。肠组织中,HSPB7和HSPB11在盐度渐变低盐胁迫(盐度变化速率1.1/h)下表达量均显著升高, HSPB1表达量在48 h显著降低;盐度骤变低盐胁迫(盐度变化速率27/h)下HSPB1和HSPB7表达量在24 h显著升高, HSPB11表达量显著降低。肾组织中,HSPB1、HSPB7和HSPB11表达量均仅在盐度渐变低盐胁迫24h显著升高;盐度骤变低盐胁迫下HSPB1表达量显著降低, HSPB7和HSPB11表达量则显著升高。肝组织中, HSPB7无表达;HSPB1表达量在盐度渐变低盐胁迫下无显著变化,但在盐度骤变低盐胁迫下则显著升高;HSPB11表达量在两种处理下均显著升高。本研究比较分析了HSPB1、HSPB7和HSPB11基因在松江鲈应对不同低盐胁迫时表达变化规律的异同,相关结果为探讨小分子热休克蛋白在鱼类应激调节过程中的作用及洄游性鱼类适应盐度变化的分子调控机制提供了理论依据。 The aim of this study was to explore the role of HSPB1, HSPB7, and HSPB11 in stress-responsive regulation of roughskin sculpin(Trachidermus fasciatus) under low salinity stress. Firstly, sequences of these genes were obtained from transcriptome data of T. fasciatus, and phylogenetic analysis was performed. In this study, fish were subjected to two different acute osmotic treatments(salinity changing rate at 27 and 1.1 ppt/h), expression patterns of the three HSPB genes in the four target tissues(gills, intestines, kidney, and liver) were examined using qRT-PCR. Phylogenetic analysis revealed that HSPB1, HSPB7, and HSPB11 genes respectively formed an independent cluster, in which T. fasciatus HSPB1, HSPB7, and HSPB11 protein shared high identity with those of Perciformes, Cypriniformes, and Cyprinodontiformes species, teleost HSPB1, HSPB7, and HSPB11 proteins formed a single lineage distinct from those of other vertebrates. Tissue-specific gene expression patterns in all three target genes showed that each tissue had a specific gene expression pattern in response to salinity changes. In the gills, the expression of HSPB1, HSPB7, and HSPB11 increased significantly at 12 h, but different expression patterns were identified in the other tissues. In the intestines, the expression of HSPB1 decreased significantly at 48 h under the relative chronic salinity stress, while the expression of HSPB7 and HSPB11 significantly increased;under the acute salinity stress, mRNA levels of HSPB1 and HSPB7 increased significantly at 24 h, and the expression of HSPB1 significantly decreased. In the kidney, expression of HSPB1, HSPB7, and HSPB11 was significantly upregulated at 24 h in response to the relative chronic salinity stress;under the acute salinity stress, the expression of HSPB1 decreased significantly, but the expression of HSPB7 and HSPB11 were significantly upregulated. The expression of HSPB7 was not detected in the liver;no significant difference in HSPB1 expression was detected under chronic salinity stress, but a signi

关 键 词： 松江鲈 小分子热休克蛋白 盐度胁迫 基因表达

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