作　　者： ; ; ;

机构地区： 佛山市顺德区慢性病防治中心

出　　处： 《中华皮肤科杂志》 2020年第5期345-351,共7页

摘　　要： 目的分析酒渣鼻患者鼻部蠕形螨寄生与鼻部皮肤微生物群落的关系。方法2017年5月至2019年6月于佛山市顺德区慢性病防治中心皮肤科收集酒渣鼻患者与面部健康对照者各14例,酒渣鼻患者中早期8例,中期6例。采集受试者鼻翼和鼻唇沟皮肤微生物样品,提取DNA,采用宏基因组测序和生物信息学分析。以蠕形螨及微生物reads数的构成比反映菌种相对丰度。计算Shannon指数评估微生物α多样性。分析基于菌种相对丰度的主成分(PCA)以评估β多样性。计数资料的比较采用两独立样本t检验,蠕形螨与微生物含量间关系采用Pearson相关性分析。结果酒渣鼻组鼻部皮肤蠕形螨相对含量(1.647%±0.389%)高于健康组(0.448%±0.089%,t=2.92,P=0.007)。蠕形螨的相对含量与细菌相对含量呈负相关(r=-0.95,P<0.001),与真菌相对含量呈正相关(r=0.76,P<0.001)。酒渣鼻组鼻细菌、真菌群落Shannon指数(0.91±0.17、1.261±0.045)显著高于健康组(0.47±0.12、0.549±0.071,t=2.17、8.48,P<0.05);两组的主成分分析结果示,仅细菌群落显著不同(t=2.32,P=0.029),而真菌群落无差异(t=0.82,P=0.461)。此外,中期酒渣鼻患者蠕形螨相对含量显著高于早期(t=6.56,P<0.001);早、中期患者中细菌和真菌的Shannon指数差异无统计学意义(均P>0.05),主成分分析结果示细菌和真菌的群落结构差异均有统计学意义(均P<0.05)。结论蠕形螨在鼻部皮肤的寄生可能影响鼻部皮肤微生态群落结构。 Objective To investigate relationships between nasal parasitism by Demodex mites and nasal skin microbiome in patients with rosacea.Methods From May 2017 to June 2019,14 patients with rosacea,including 8 with early-stage rosacea and 6 with intermediate-stage rosacea,and 14 human controls with healthy facial skin were collected from Department of Dermatology,Shunde District Center for Prevention and Cure of Chronic Disease of Foshan City.Microbial samples were collected from the nasal alar and nasolabial folds of the subjects.Then,DNA was extracted from the samples,and subjected to metagenomic sequencing and bioinformatics analysis.Relative abundance of strains was estimated by using composition ratios of Demodex mites and microbial reads.Shannon index was calculated to evaluateαdiversity of microbiome,and principal component analysis(PCA)was performed to assessβdiversity based on relative abundance of microbial species.Enumeration data were compared by using two-independent-sample t test,and relationships between nasal Demodex mites and skin microbiome were analyzed by Pearson correlation analysis.Results The relative abundance of nasal Demodex mites was significantly higher in the rosacea group(1.647%±0.389%)than in the healthy group(0.448%±0.089%,t=2.92,P=0.007).The relative abundance of Demodex mites was negatively correlated with the relative abundance of bacteria(r=-0.95,P<0.001),and positively correlated with the relative abundance of fungi(r=0.76,P<0.001).The Shannon indices of nasal bacterial and fungal communities were significantly higher in the rosacea group(0.91±0.17,1.261±0.045,respectively)than in the healthy group(0.47±0.12,0.549±0.071,t=2.17,8.48,respectively,both P<0.05);PCA showed that theβdiversity of bacterial communities significantly differed between the rosacea group and healthy group(t=2.32,P=0.029),while no significant difference in theβdiversity of fungal communities was observed between the two groups(t=0.82,P=0.461).In addition,the relative abundance of Demodex mites was sign

关 键 词： 红斑痤疮 宏基因组学 蠕形螨 微生态 菌群失调

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