作　　者： ;

机构地区： 中南大学湘雅医学院基础医学院

出　　处： 《现代生物医学进展》 2018年第1期78-81,116,共5页

摘　　要： 目的:探讨缺血性心肌病(ICM)患者血浆microRNA-21(miR-21)的表达及作用机制。方法:选取我院2010年2月-2016年3月收治的54例ICM患者为观察组,选取同期58例健康体检者为对照组,运用实时荧光定量PCR(q RT-PCR)测定两组血浆miR-21表达水平,比较两组受试者总胆固醇(TC)、三酰甘油(TG)、左心室射血分数(LVEF)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、左心室舒张末期容积(LVEDV)、N末端B型脑钠肽原(NT-proBNP)的变化,分析血浆miR-21水平与以上各项临床指标的相关性,评估miR-21在ICM诊治中的价值。结果:观察组LDL-C、HDL-C、LVEF水平低于对照组,差异有统计学意义(P<0.05),血浆miR-21、TG、NT-proBNP及LVEDV水平高于对照组,差异有统计学意义(P<0.05)。经Pearson相关分析显示,观察组血浆miR-21水平与NT-proBNP和LVEDV呈正相关(P<0.05),而血浆miR-21水平与LVEF、LDL-C、HDL-C、TG、TC无相关性(P>0.05)。结论:ICM患者血浆miR-21表达明显升高,并且其表达与NT-proBNP及LVEDV呈正相关,通过参与ICM患者心室重构发挥作用,可作为ICM诊治的新靶点及风险评估的新生物学标志物。 Objective： To investigate the expression and mechanism of plasma microRNA-21 （miR-21） in patients with ischemic cardiomyopathy （ICM）. Methods： 54 patients with ICM who were treated in our hospital from February 20 10 to March 2016 were selected as the observation group, 58 healthy subjects were selected as the control group at the same time.The expression level of miR-21 of the two groups were measured by real-time quantitative PCR （qRT-PCR）. The differences of total cholesterol （TC）,three glycerol （TG）, left ventricular ejection fraction （LVEF）, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C）, left ven- tricular end diastolic volume （LVEDV）, N terminal B type brain natriuretic peptide （NT-proBNP） of the two groups were compared. The correlation between plasma miR-21 level and the above clinical parameters was analyzed, and the value of miR-21 in ICM diagnosis and treatment was evaluated. Results： The levels of LDL-C, HDL-C and LVEF in the observation group were lower than those in the control group, the difference was statistically significant （P〈0.05）. The plasma levels of miR-21, TG, NT-proBNP and LVEDV were higher than those of the control group,the difference was statistically significant （P〈0.05）. Pearson correlation analysis showed tb.at the plasma level ofmiR-21 in the observation group were positively correlated with NT-proBNP and LVEDV （P〈0.05）. The plasma level ofmiR-21 were not correlated with LVEF, LDL-C, HDL-C, TG and TC （P〉0.05）. Conclusion： The expression of plasma miR-21 is increased significantly in patients with ICM, and the expression is positively correlated with NT-proBNP and LVEDV. It plays a role in ventricular remodeling oflCM patients, which can be used as a new target for the diagnosis and treatment of ICM and a new biomarker for risk assessment.

关 键 词： 心室重构 末端 型脑钠肽原 缺血性心肌病

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