作　　者： (）;

机构地区： 华南农业大学电子工程学院

出　　处： 《物理实验》 2018年第4期16-19,23,共5页

摘　　要： 基于荧光淬灭原理,利用光纤氧气传感器检测PK-15细胞培养过程中的氧分压.实验结果表明:随着细胞密度的增高,测得的氧分压减小,说明细胞在代谢过程中,耗氧量要大于氧气的扩散溶解量.细胞培养过程中液相中的氧分压水平不仅依赖于细胞培养环境气相中的氧分压,而且也依赖于细胞的密度和细胞代谢. Based on fluorescence quenching principle,the oxygen partial pressure in the PK-15 cell culture was detected using fiber optic oxygen sensor.The results showed that the measured data of oxygen partial pressure decreased when the cell density increased,indicating that in the metabolism process,the amount of dissolved oxygen consumption should be greater than that of the diffused of oxygen.The level of oxygen partial pressure in the liquid phase of cell culture not only depended on the oxygen partial pressure,but also depended on the cell density and cell metabolism.

关 键 词： 氧分压 光纤氧气传感器 荧光淬灭 细胞培养

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