作　　者： (）;

机构地区： 广州中医药大学中医药数理工程研究院

出　　处： 《中华中医药杂志》 2018年第9期4089-4092,共4页

摘　　要： 目的:利用DNA条形码技术分析比较姜科植物的ycf1序列,对姜科药用植物进行条形码鉴定及聚类分析,评价ycf1序列对姜科植物的鉴别能力。方法:用ycf1通用引物对姜科6个属16种药用植物进行PCR扩增和测序,用Bio Edit v7.0.9.0及MEGA 6.0对目的序列进行比对分析,以美人蕉为外类群构建系统进化树。结果:姜科ycf1序列长度约为900bp,17条目的序列差异位点150个,除山姜属4个种的种间遗传距离为0无法鉴别以外,其余5个属种间均存在序列差异;构建的系统进化树显示,ycf1序列能明显区分姜科6个属的植物。结论:ycf1序列可对姜科植物进行可靠的遗传聚类分析,且可对本研究中除山姜属4个近源种以外的姜科植物进行鉴定。 Objective： The DNA barcode technique was used to analyze the ycf1 sequence of Zingiberaceae plants, and the barcode identification and cluster analysis of the medicinal plants of Zingiberaceae were conducted to evaluate the identification ability of ycf1 sequence on Zingiberaceae plants. Methods： Sixteen medicinal plants of 6 genera of Zingiberaceae were amplified and sequenced by ycf1 universal primers. The target sequences were analyzed by Bio Edit v7.0.9.0 and MEGA 6.0, and the Canna indica Linnaeus was used as the outer group to construct the phylogenetic tree. Results： The length of the ycf1 sequence of Zingiberaceae was about 900 bp, and the sequence difference sites of 17 entries were 150. Except for the genetic distance between the four species of the Alpinia, which was 0, the other five genera had sequence differences. The constructed phylogenetic tree showed that the ycf1 sequence clearly distinguishes the plants of the six genera of the Zingiberaceae. Conclusion： The ycf1 sequence can be used for reliable genetic cluster analysis of Zingiberaceae plants, and the four species of the Alpinia, the Zingiberaceae plants can be identified in this study.

关 键 词： 姜科 分子鉴定 聚类分析 条形码技术

领　　域： []