作　　者： ; ; ; ;

机构地区： 南方医科大学

出　　处： 《中国医药导报》 2018年第16期17-20,182,共5页

摘　　要： 目的探讨长链非编码RNA-ANRIL(lncRNA ANRIL)在乳腺癌细胞增殖、侵袭和凋亡中的作用及相关机制。方法在乳腺癌细胞中瞬时转染ANRIL小干扰RNA片段(si-ANRIL),采用Real-time PCR检测干扰效果,MTT法检测降低ANRIL表达对细胞增殖能力的影响,Transwell实验检测降低ANRIL表达对细胞侵袭能力的影响,流式细胞术(FCM)检测降低ANRIL表达对细胞凋亡能力的影响,Western blot检测降低ANRIL表达对p53蛋白表达的影响;在乳腺癌细胞中瞬时共转si-ANRIL和p53小干扰RNA片段(si-p53),采用MTT、Transwell、FCM等方法检测乳腺癌细胞增殖、侵袭和凋亡的改变。结果干扰ANRIL表达,乳腺癌细胞增殖减慢,侵袭迁移能力减弱,凋亡率显著升高(P<0.05),p53通路被激活;同时干扰p53可部分逆转ANRIL表达下降所致细胞增殖、侵袭能力下降和凋亡能力增强。结论 lncRNA ANRIL可通过抑制p53信号通路促进乳腺癌细胞增殖、侵袭,抑制凋亡,进而促进乳腺癌发生发展。 Objective To explore the role and mechanism of long noncoding RNA-ANRIL（lncRNA ANRIL） in proliferation,invasion and apoptosis of breast cancer cells.Methods Breast cancer cells were transiently transfected with siANRIL.Real-time PCR was used to confirm the interfere effect of si-ANRIL,MTT assay was used to detect cell proliferation,transwell method was used to detect cell invasion,flow cytometer（FCM） was used to detect cell apoptosis,Western blot was used to detect the protein expression level of p53.Breast cancer cells were co-transfected with si-ANRIL and si-p53,MTT assay,transwell method and FCM were used to detect cell proliferation,invasion and apoptosis.Results ANRIL knockdown decreased cell proliferation,invasion,and increased cell apoptosis（P〈0.05）.ANRIL knockdown activated p53 signal pathway.Simultaneous p53 knockdown partially reversed the effect of ANRIL knockdown.Conclusion Lnc RNA ANRIL can promote the proliferation,invasion and inhibit apoptosis of breast cancer cells by inhibiting p53 pathway,thus promoting the occurrence and development of breast cancer.

关 键 词： 长链非编码 乳腺癌 增殖 侵袭 凋亡

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