作　　者： ; ; ; ;

机构地区： 深圳市福田区疾病预防控制中心

出　　处： 《疾病监测》 2019年第1期80-83,共4页

摘　　要： 目的调查B群流行性脑脊髓膜炎(流脑)疫情的原因。方法于2018年2月22、27日对患者和密切接触者进行流行病学调查,并收集脑脊液、血液和咽拭子样品进行分离培养、PCR检测和二代测序。结果在患者血液及其母亲咽拭子中均分离出B群脑膜炎奈瑟菌,多位点序列分型均为ST-3200/CC4821,2株菌的16S rRNA及耐药基因、多肽编码基因的相似性为100%。结论该疫情是由健康携带B群脑膜炎奈瑟菌的母亲传播给患者而引起。 Objective To investigate the cause of a serogroup B meningococcal meningitis case in Shenzhen.Methods The epidemiological investigation was conducted for the case and close contacts.The cerebrospinal fluid and blood samples from the case and the throat swabs from the contacts were collected for the pathogen isolation/culture and PCR detection.The isolates were sequenced with next generation sequencing method.Results Two strains of serogroup B Neisseria meningitidis were isolated from the blood sample of the case and throat swab of case’s mother,respectively.The two isolates shared the same multilocus sequence typing(MLST) type of ST-3200/CC4821,same 16 S rRNA sequence,and same alleles of genes related to antibiotic susceptibility and coding peptides.Conclusion The meningococcal disease case was caused by the transmission of serogroup B N.meningitidis from case’ mother who was a healthy carrier.

关 键 词： 流行性脑脊髓膜炎 群脑膜炎奈瑟菌 流行病学调查 二代测序

领　　域： []