作　　者： ; ;

机构地区： 广州中医药大学

出　　处： 《新中医》 2019年第6期5-7,共3页

摘　　要： 目的:探讨温阳活血方对慢性心力衰竭(Chronic heart failure,CHF)大鼠心室重构的影响。方法:用肾上腹主动脉缩窄法制备CHF模型。雄性Wistar大鼠30只随机分为假手术组、模型组、温阳活血方组,每组10只。观察各组大鼠左心室重量指数(Left ventricular mass index,LVMI)、胶原容积分数(Collagen volume fraction,CVF),免疫组化法测定各组大鼠左心室心肌结缔组织生长因子(Connective tissue growth factor,CTGF)、转化生长因子(Transforming growth factor-β1,TGF-β1) mRNA表达水平。结果:与假手术组比较,模型组LVMI、CVF明显升高(P <0.05),左心室心肌CTGF、TGF-β1 mRNA表达明显升高(P <0.01);与模型组比较,温阳活血方组LVMI、CVF明显下降(P <0.05),左心室心肌CTGF、TGF-β1 mRNA表达明显下降(P <0.01)。结论:温阳活血方通过影响信号通路中CTGF、TGF-β1 mRNA的表达来改善心衰大鼠心肌纤维化(Myocardial fibrosis,MF)程度。 Objective:To discuss the effect of yang-warming and blood-promoting prescription on ventricular remodeling in rats with chronic heart failure(CHF).Methods:Prepared the CHF model by suprarenal abdominal aortic coarctation method.Divided 30 cases of male Wistar rats randomly into the sham operation group,the model group and the group of yang-warming and blood-promoting prescription,10 rats in each group.Observed the left ventricular mass index(LVMI)and collagen volume fraction(CVF)of rats in each group,and detected the left ventricular myocardial connective tissue growth factor(CTGF)and the expression level of transforming growth factor-β1(TGF-β1)m RNA by immunohistochemistry.Results:Compared with that in the sham operation group,the LVMI and CVF in the model group were significantly increased(P<0.05);the expressions of CTGF and TGF-β1 mRNA in the left ventricle were significantly increased(P<0.01).Compared with that in the model group,the LVMI and CVF in the group of yang-warming and blood-promoting prescription were decreased evidently(P<0.05);and the CTGF and the expression of TGF-β1 mRNA in left ventricle myocardium were obviously decreased(P<0.01).Conclusion:The application of yang-warming and blood-promoting prescription can improve the degree of myocardial fibrosis(MF)in rats with heart failure by affecting the CTGF and the expression of TGF-β1 mRNA in the signaling pathway.

关 键 词： 慢性心力衰竭 温阳活血方 心室重构 动物实验 大鼠

领　　域： []