作　　者： (）;

机构地区： 佛山市妇幼保健院

出　　处： 《国际泌尿系统杂志》 2019年第5期844-847,共4页

摘　　要： 目的研究精子自发顶体反应率与精子存活率、总活动力及畸形率的关系,探讨精子自发顶体反应对男性生育的影响。方法收集2017年12月至2018年5月在佛山市妇幼保健院生殖中心就诊的男性患者精液标本共223份。患者禁欲2-7d,收集全部精液于一次性无菌、无毒容器中。以WHO第五版《人类精液检查与处理实验室手册》为标准,采用CASA系统进行精液的常规分析,计算精子存活率、总活动力和染色观察精子畸形率。采用PSA-FITC染色法进行精子自发顶体反应试验。按照顶体反应(AR)阳性率>10为异常,分AR>10、AR<10两组,对精子自发顶体反应率与精子存活率、总活动力及畸形率之间的关系进行统计分析。结果精子自发顶体反应正常组与异常组这两组间的精子存活率和总活动力差异有统计学意义(P<0.05)。精子自发顶体反应率与精子存活率和总活动力差异有统计学意义(r=-0.249,P<0.05;r=-0.256,P<0.05)。精子自发顶体反应率与精子畸形率差异无统计学意义(P>0.05)。结论精子自发顶体反应率与精子的总活动力及存活率有关,精子自发顶体反应可作为评价男性生育功能的指标之一。 Objective To study the exploring of correlationship between hunm an spermatozoa spontaneous acrosome reaction rate and sperm survival rate and total motility and abnormal rate and to evaluate the effect of hunman spermatozoa spontaneous acrosome reaction rate on male fertility. Methods A total of 223 semen sam ples in our hospital from December 2017 to May 2018 were selected. The patient was abstinent for 2 - 7 d ay s, and all semen was collected in a disposable sterile and nontoxic container. All the standards were from WHO laboratory manual for the examination and processing of human sperm ( fifth ed itio n ). Routine analysis of semen was performed using the CASA system. Sperm survival rate and total motility were calculated. The sperm abnormality rate after papanicolaou staining was observed. The spermatozoa spontaneous acrosome reaction rate was tested by PSA-FITC staining. According to the positive rate of acrosome reaction ( AR ) than 10 was abnorm al, two groups were divided into AR > 10 and AR no more than 10. Correlationship between hunman spermatozoa spontaneous acrosome reaction rate and sperm survival rate and total motility and abnormal rate were explored by SPSS 22. 0 statistical software. Results There were significant differences in sperm survival rate and total motility between normal and abnormal g ro u p s(P < 0 . 0 5 ). The hunman spermatozoa spontaneous acrosome reaction rate was negatively correlated with sperm survival rate and total motility ( r =- 0. 249, P < 0. 05;r =- 0. 256, P < 0. 05). There was no significant correlation between hunman spermatozoa spontaneous acrosome reaction rate and sperm abnormal rate. Conclusions The hunman spermatozoa spontaneous acrosome reaction rate is related the sperm survival rate and total m otility, and it can be used as one of the indicator of male reproductive function.

关 键 词： 精液 顶体反应 存活率 畸形精子症

领　　域： []