作　　者： ;

机构地区： 广州中医药大学

出　　处： 《中国实验方剂学杂志》 2017年第23期109-115,共7页

摘　　要： 目的：探讨消脂汤对游离脂肪酸（FFA）诱导的非酒精性脂肪性肝炎（NASH）细胞模型的作用及机制。方法：用0.5μmol·L-1FFA诱导Hep G2细胞建立NASH细胞模型,用消脂汤及非诺贝特干预NASH细胞模型;油红O染色观察细胞内脂滴含量;酶联免疫吸附试验（ELISA）检测细胞上清液中肿瘤坏死因子-α（TNF-α）,白细胞介素-6（IL-6）含量;蛋白免疫印迹法（Western blot）检测核转录因子-κB（NF-κB）p65,IκB激酶（IKKβ）,IκB磷酸化激酶[Phospho-IKKα/β（Ser176/180）],胰岛素受体底物1磷酸化蛋白[Phospho-IRS-1（Ser^307）],胰岛素受体底物-1（IRS-1）蛋白表达。结果：FFA刺激24 h后,模型组内脂滴含量,细胞上清液中TNF-α,IL-6含量高于正常组（P〈0.01）,IKKβ,p-IKKα/β（Ser176/180）,p-IRS-1（Ser^307）,NF-κB（核蛋白）表达明显增加（P〈0.01）,IRS-1蛋白表达减少（P〈0.05）,各给药组p-IKKα/β（Ser176/180）,p-IRS-1（Ser^307）,NF-κB p65（核蛋白）表达减少（P〈0.01）,尤其是消脂汤高浓度组较非诺贝特p-IKKα/β（Ser176/180）,p-IRS-1（Ser^307）蛋白表达显著减少（P〈0.01）,各给药组IRS-1蛋白表达明显增加（P〈0.05）。结论：消脂汤可改善FFA诱导的NASH细胞模型中脂质沉积及炎症反应,其机制可能与抑制细胞内IKKα/β,IRS-1 Ser^307的磷酸化及IKKβ,NF-κB p65（核蛋白）表达有关。 Objective： To investigate the effect and mechanism of Xiaozhi decoction on free fatty acids （FFA） -induced non-alcoholic steatohepatitis （NASH） cell model. Method： The NASH model in HepG2 cells was induced by 0.5 μmol·L^-1 FFA. The NASH cell models were established by different concentrations of Xiaozhi decoction and fenofibrate. Oil red O staining was used to observe the content of lipid droplets. Cell supernatant （nuclear faetor-κ-gene binding） NF-κB p65, IkappaB kinase beta （IKKβ）, Phospho-IκB kinase alpha/beta [ Phospho-IKKα/β （ Ser176/180）], Phospho-insulin receptor substrate [ Phospho-IRS-1 （ Ser307） ] and insulin receptor substrate-1 （IRS-1） protein were detected by Western blot. Result： The contents of tumor necrosis factor-alpha （TNF-α） and interleukin-6 （IL-6） in the supernatant fluid of the model group were significantly higher than those in control group （P 〈 0.01 ）. Meanwhile, the expression of IRS-1 was reduced （P 〈 0.05 ）, while the levels of IKKβ, p-IKKα/β （Ser176/180） and IRS-1 （Ser307） phosphorylation and nuclear translocation of NF-κB p65 protein were increased in the model group （P 〈 0.01 ）. The expressions of IKKα/β （ Ser176/180） and IRS-1 （ Ser307 ） phosphorylation and nuclear translocation of NF-κB p65 protein in each dose group were decreased （P 〈 0.01 ） , especially in high-dose Xiaozhi decoction. The protein expression of IRS-1 was significantly increased （P 〈 0.05） in control group. Conclusion： Xiaozhi decoction can improve lipid deposition and inflammatory reaction in NASH cell model induced by FFA. The mechanism may be related to the inhibition of intracellular IKKα/β （Ser176/180） and IRS-1 （Ser307） phosphorylation and IKKβ, NF-κB nuclear protein expression.

关 键 词： 非酒精性脂肪性肝炎 HEP G2细胞模型 消脂汤 游离脂肪酸 核转录因子-κB IκB抑制蛋白激酶α/β 胰岛素受体底物-1

分 类 号： [R285.5]

领　　域： []