作　　者： (赵雪）; (杨云霜）; (张蓉）; (匡振坤）; (杜磊）; (石鹏展）; (余雷）;

机构地区： 锦州医科大学火箭军总医院研究生培养基地,121000

出　　处： 《中华放射医学与防护杂志》 2017年第8期565-569,共5页

摘　　要： 目的 探讨补肾解毒方对电离辐射致小鼠性激素紊乱的防治作用。方法 72只成年小鼠雌雄各半采用随机数表法分为6组，每组12只，分别为雌、雄性的空白对照组、辐射组、补肾解毒方组。适应性饲养2 d后，补肾解毒方组给予浓度1.38 g/ml的补肾解毒方水溶颗粒剂0.2 ml灌胃，1次/d，灌胃10 d，第11天给予辐射组、补肾解毒方组一次性剂量为5 Gy的60Co γ射线照射，照射后24 h，各组采用随机数表法抽取6只处死，检测性激素水平；补肾解毒方组继续予补肾解毒方灌胃1次/d，灌胃14 d，空白对照组与辐射组正常饮水饮食饲养，照射后第14天，其余小鼠处死取材，检测性激素水平。结果 辐射后第1天，雌鼠补肾解毒方组雌二醇（E2）升高，与雌鼠辐射组相比，差异有统计学意义（t=2.686，P〈0.01）；雌、雄鼠辐射组卵泡雌激素（FSH）水平与空白对照组相比均明显升高（t=4.305、4.642，P〈0.01）；雄鼠辐射组、补肾解毒方组黄体生成素（LH）水平升高，与雄鼠空白对照组相比，差异有统计学意义（t=4.537、2.336，P〈0.01）。辐射后第14天，雄鼠睾酮（T）、LH辐射组低于空白对照组（t=-3.730、2.963，P〈0.05）；雌鼠辐射组、补肾解毒方组E2、孕激素（P）下降，明显低于空白对照组（t=-5.424、-3.178，-2.690、-3.215，P〈0.01）；雄、雌鼠辐射组FSH与空白对照组差异有统计学意义（t=-3.905、-2.812，P〈0.05）。结论 电离辐射会导致性激素分泌紊乱，而补肾解毒方能有效抑制该情况的产生，其对电离辐射引起的性激素紊乱具有较好的防护作用。 Objective To investigate the preventive and therapeutic effect of Bushen Jiedu prescription on ionizing radiation induced sex hormone disorder. Methods Totally 72 adult rats with half male and half female were randomly divided into 6 groups, including control group, radiation group, Bushen Jiedu prescription group with male and female, with 12 rats in each group. After adaptive breeding for 2 d, the rats of Bushen Jiedu prescription group were given 0.2 ml water soluble granules of Bushen Jiedu prescription （1.38 g/ml） by gavage once a day for 10 days. At 11 d, the rats were irradiated by 5 Gy of 60Co γ-rays. 24 h after radiation, 6 rats in each group were executed to measure the level of sex hormone. The other rats were further gavaged with Bushen Jiedu prescription or its control once a day for 14 d, then were executed for sex hormone testing. Results At 1 d after radiation, the estrogen （E2） of female of Bushen Jiedu prescription group was significantly higher than that of radiation group （t=2.686, P〈0.01）. Compared with control group, the levels of follicule-stimulating hormone （FSH） （t=4.305, 4.642, P〈0.01） of male and female, the levels of luteinizing hormone （LH） of male radiation group, and Bushen Jiedu prescription group were increased （t=4.537, 2.336, P〈0.01）. At 14 d after radiation, the levels of testosterone and luteinizing hormone in male rats of radiation group was higher than those in control group （t=-3.730, 2.963, P〈0.05）, and the levels of E2 and progesterone of female was lower than those in control group （t=-5.424, -3.178, -2.690, -3.215, P〈0.01）. Conclusions Ionizing radiation can induced sex hormone disorder, and Bushen Jiedu prescription can effectively inhibit this alteration, which indicates that Bushen Jiedu prescription is an ideal radiation protectant.

关 键 词： 电离辐射 补肾解毒方 性激素