作　　者： (王海军）; (王善辉）; (昌莉丽）; (陈鸿军）;

机构地区： 徐州生物工程职业技术学院,徐州221006

出　　处： 《中国动物传染病学报》 2017年第4期64-67,共4页

摘　　要： 为建立检测牛传染性鼻气管炎病毒(Bovine infectious rhinotracheitis virus,IBRV)gB基因的荧光定量PCR检测方法,本研究根据gB基因序列,设计1对引物和相应的TaqMan探针。建立和优化反应体系后,以10倍稀释的病毒来检测该方法的灵敏度。同时,对伪狂犬病毒(Pseudorabies virus,PRV)、马立克病病毒(Marek’s disease virus,MDV)和鸭瘟病毒(Duck plague virus,DPV)进行特异性检测。结果表明,基于gB基因的荧光PCR检测方法可用于鉴定IBRV,该方法具有较好的灵敏度和特异性,其灵敏度为10 copies/μL,即0.02 TCID_(50),且与其他病毒无交叉反应。 In order to detect infectious Bovine rhinotracheitis virus（IBRV）, a TaqMan fluorescence quantitative PCR detection method was developed using specifically paired primers and corresponding TaqMan probe targeting to the gB gene of IBRV. After establishing and optimizing the reaction system, the sensitivity of the method was detected by a 10-fold dilution of the virus. At the same time, specific detection of Pseudorabies virus（PRV）, Marek＇s disease virus（MDV） and Duck plague virus（DPV） was conducted. Results showed that the fluorescence PCR detection method based on gB gene can be used to identify IBRV, which has good sensitivity and specificity, and the sensitivity of 10 copies/u L, namely 0.02 TCID_（50）, and no cross reaction with other virus.

关 键 词： 牛传染性鼻气管炎病毒 实时荧光定量