作　　者： (曹丽梅）; (赵萱）; (李杰）; (何瑶）; (傅超美）;

机构地区： 成都中医药大学药学院,成都611137

出　　处： 《中药与临床》 2017年第2期45-48,共4页

摘　　要： 目的:研究人参附子药对不同配比对慢性心力衰竭大鼠心肌细胞凋亡的影响,优选参附注射液中人参-附子药对改善慢性心力衰竭的最佳比例,阐明参附药对配伍与疗效的关系。方法:采用腹腔注射盐酸阿霉素(ADR)法建立大鼠慢性心力衰竭(CHF)模型,测定大鼠体重及心脏重量指数(HWI)的变化,HE染色后观察大鼠心肌组织切片病理损伤情况,通过实时荧光定量PCR(qRT-PCR)法检测其对抗凋亡基因(Bcl-2)、促凋亡基因(Bax)、细胞色素C(Cytc)、半胱氨酸天冬氨酸蛋白酶9(Caspase-9)和半胱氨酸天冬氨酸蛋白酶3(Caspase-3)基因mRNA表达水平的影响。结果:参附注射液组与其药对不同配比组均能不同程度降低慢性心衰大鼠心脏重量指数,解缓心肌冲血程度,上调抗凋亡基因(Bcl-2)表达,下调促凋亡基因(Bax)、细胞色素C(Cytc)、Caspase-9和Caspase-3的表达,抑制线粒体途径心肌细胞的凋亡,其中,以人参-附子1:2配比组作用最强。结论:人参与附子配伍比例为1:2时,抗慢性心力衰竭线粒体途径心肌细胞凋亡的效果最佳,其可能是通过抑制Bcl-2的表达,上调Bax基因表达,来抑制细胞色素C(Cytc)释放,从而降低Caspase-9和Caspase-3的水平,最终抑制慢性心力衰竭大鼠心肌细胞凋亡,保护心肌,改善心功能。 Objective： To study the effect of different dosage ration of Renshen and Fuzi on myocardial apoptosis in rat with chronic heart failure （CHF）, explore the best dosage ration of Renshen and Fuzi in Shenfu Injection and clarify the relationship between compatibility and efficiency. Method： CHF model was established by intraperitoneal injection of doxorubicin hydrochloride （ADR）. Weight and heart weight index （WHI） were measured. Cardiac muscle tissue slice pathological injury were observed after HE. The indexes including Bcl-2, Bax, CytC, Caspase-9 and Caspase-3 were measures by qRT-PCR. Result： Shenfu Injection and different dosage ration of Renshen -Fuzi can decrease WHI of CHF rats, relieve myocardial ischemia in different level. They can also increase the expression of antiapoptotic genes （Bcl-2）, decline the express of apoptosis gene （Bax）, Cytc, Caspase 9, and Caspase 3 to inhibit mitochondrial pathway of myocardial cell apoptosis. Among them, Renshen-Fuzil：2 group showed the strongest effect. Conclusion： The effect of Renshen -Fuzi 1：2 group on resistance to chronic heart failure mitochondrial pathway of myocardial cell apoptosis is the best. It is probably by inhibiting the expression of Bcl-2, raising Bax gene expression, to suppress the cytochrome C （Cytc） release, thus reduce Caspase - 9 and Caspase - 3 levels, eventually inhibit myocardial apoptosis in chronic heart failure rats and protect cardiac muscle and improve heart function.

关 键 词： 人参 附子 药对 慢性心力衰竭 心肌细胞凋亡