作　　者： (高原）; (丁万隆）; (李勇）;

机构地区： 中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处： 《中国现代中药》 2017年第8期1165-1169,共5页

摘　　要： 目的:研究人参锈腐菌Cylindrocarpon destructans接种后人参根尖显微及超微结构的变化情况。方法:采用石蜡切片法研究人参根尖显微结构,透射电镜研究人参根尖超微结构。结果:与对照相比,锈腐菌接种7 d后人参根尖表皮细胞细胞壁明显增厚,表皮细胞内出现颗粒物沉积,部分表皮细胞的细胞壁出现破裂,细胞发生解体,且根内髓部同样出现颗粒物。超微结构观察发现,对照组人参根尖细胞形状规则,内含物丰富,细胞器清晰可辨;接种人参锈腐菌后人参根尖细胞形态异常,细胞内含物减少,细胞器因解体消失而无法辨别。结论:人参锈腐菌可能是先侵染人参表皮细胞,再通过细胞间隙进入木质部,阻碍水分和无机盐运输,导致人参发病死亡。 Objective：To study the microstructure and ultrastructure changes of Panax ginseng roots infected by Cylindrocarpon destructans.Methods：The paraffin section was used to observe the microstructure of root tips of P.ginseng.The superfine slice was used to observe the ultrastructure of root tips of P.ginseng.Results：Microstructure showed that the morphology and structure of epidermal cells of P.ginseng were regular in control.However，epidermal cell wall became thicker 7 days post inoculation of C.destructans，and particles deposition in cells as well as in pith parts was shown.The cell walls were broken，cells began to disintegrate.Ultrastructure showed that morphology of root cells of P.ginseng was regular in control.Cells had abundant contents，and organelles were easy to observe.On the contrary，morphology of cells was abnormal and contents in cells were decreased when infected by C.destructans.The organelles were break up，and some of them were disappeared.Conclusion：The hypha invaded the epidermal cells of P.ginseng maybe the first step of C.destructans infection.Then hypha entered into xylem of ginseng root through the intercellular space，which obstructed the transport of water and inorganic salt，and finally lead to the death of root cells.

关 键 词： 人参 人参锈腐菌 侵染 显微结构 超微结构