作　　者： (张皓淳）; (张鑫）; (杨作丰）; (马建山）; (邓文超）; (王冰）;

机构地区： 辽宁省动物疫病预防控制中心,辽宁沈阳110164 辽宁省动物医学研究院,辽宁沈阳110164

出　　处： 《现代畜牧兽医》 2017年第8期17-22,共6页

摘　　要： 为确诊锦州某蜂场蜜蜂大量死亡病因,本试验采用反转录-聚合酶链反应(RT-PCR)方法,收集病死蜜蜂提取总RNA,进行反转录,获得c DNA。然后,以c DNA为模板,利用检测常见蜜蜂病毒的引物进行PCR扩增。结果表明,从蜜蜂病料中扩增出编码蜜蜂残翅病毒Helicase蛋白酶的基因片段,大小约702 bp,将分离毒株命名为DWV-JZ。测序结果通过BLAST比对以及与Gen Bank中获得的7个序列进行遗传进化分析,证实DWV-JZ与Gen Bank中的DWV-JL1(KP096414.1)同源性达到95%,亲缘关系最近。 To diagnose the cause of death of a large number of bee in Jinzhou a apiary. In this study, total RNA was extracted from dead bees, and eDNA were obtained by reverse-transcriptase PCR （RT-PCR） method. Then, using eDNA as a template, PeR amplification was performed using prim- ers for detecting common honeybee viruses. The results show, a gene fragment about 702 bp encoding deformed wing virus helicase protease was amplified from the bee material, the separation of the virus named DWV-JZ. Sequencing results were analyzed by BLAST and proceeded phylogenetie analysis with seven reference sequences obtained in GenBank. It was confirmed that DWV-JZ had 95% ho- mology with DWV-JLl（KP096414.1） in GenBank and had the closest genetic relationship.

关 键 词： 蜜蜂残翼病毒 诊断 遗传进化分析