作　　者： (赵志强）; (薛满德）; (黄珂）; (张静文）; (龙艳）; (裴新梧）; (袁潜华）;

机构地区： 海南大学热带农林学院,海口570228

出　　处： 《生物技术通报》 2017年第8期51-57,共7页

摘　　要： 绿色组织特异表达启动子可调控外源基因只在受体作物的绿色组织中定点、高效地表达。以普通野生稻为实验材料,克隆了绿色组织特异表达启动子Or GSP,构建Or GSP和GUS基因融合的表达载体,转入拟南芥中鉴定功能。启动子Or GSP长度为825 bp,含有基本的转录起始元件TATA-box和CAAT-box,以及光响应元件TCCC-motif、Sp1、G-box、I-box、GA-motif和as-2-box等。转基因拟南芥GUS组织化学染色结果表明,启动子Or GSP调控GUS基因只在绿色组织中特异表达。GUS活性测定结果显示,叶和茎中的GUS活性比根中明显提高。普通野生稻中克隆的启动子Or GSP为绿色组织特异表达启动子,可为作物分子育种提供新的调控元件。 Green tissue-specific expression promoter,which makes the exogenous gene express efficiently in green tissues of receptorcrop. The green tissue-specific promoter Or GSP was cloned from Oryza rufipogon,and the fusion vector of Or GSP and GUS gene wasconstructed and transferred into Arabidopsis thaliana in order to identify the function of the promoter. Bioinformatics analysis shows that thelength of 825 bp of Or GSP,contains the basic transcription initiation elements TATA-box and CAAT-box,and light responsive elementsTCCC-motif,Sp1,G-box,I-box,GA-motif and as-2-box,etc. The GUS histochemical staining of transgenic Arabidopsis thaliana showsthat Or GSP regulates the expression of GUS gene only in green tissue,and the GUS activity in leaf and stem is significantly higher than in roots.The promoter of Oryza rufipogon,Or GSP,is a green tissue-specific promoter and the results can provide new regulatory elements for cropmolecular breeding.

关 键 词： 启动子 绿色组织特异表达 普通野生稻