作　　者： (包小红）; (何筱毅）; (刘繁红）; (周娟）;

机构地区： 四川省食品药品检验检测院,四川成都611731

出　　处： 《中药与临床》 2017年第1期29-31,共3页

摘　　要： 目的:针对九味羌活颗粒中4种成分的含量,建立超高效液相快速测定方法,有效评价九味羌活颗粒的质量。方法:采用UPLC-UV法,色谱柱为C18色谱柱,流动相为甲醇-0.1%磷酸溶液,梯度洗脱,检测波长310 nm。结果:紫花前胡苷在4.38~438 ng、阿米醇苷在0.91~91.29 ng、异欧前胡素在0.60~60.04 ng、羌活醇在1.30~130.20 ng的范围内线性关系良好(r=0.9999)。平均回收率分别为98.8%(紫花前胡苷)、97.0%(阿米醇苷)、95.6%(异欧前胡素)、102.8%(羌活醇)。结论:建立的含量测定方法专属性强、重复性好,操作简便,可以有效地评价不同样品之间的质量差异。 Objective： An ultra performance liquid chromatography method was established for the rapid determination of effective quality evaluation of four chemicals in Jiuwei Qianghuo Keli. Method： UPLC - UV method was used in the detection. The analysis was carried on C18 column with mobile phase of methanol -0.1% phosphoric acid solution in gradient elution. The wavelength for detection was set at 310 nm. Result： The linear range was 4.38- 438 ng, 0.91 -91.29 ng, 0.60 - 60.04 ng and 1.30 - 130.20 ng for nodakenin, amie glycosides, isoimperatorin and notopteol, respectively （r= 0.9999）. The average recovery was 98.8%, 97.0%, 95.6%, 102.8% for nodakenin, amie glycosides, isoimperatorin and notopteol, respectively. Conclusion： The established content determination method has strong specificity, repeatability, and convenient operability which can effectively evaluate the quality between different samples.

关 键 词： 九味羌活颗粒 紫花前胡苷 羌活醇 超高效液相色谱法