作　　者： (范丽）; (陈建婷）;

机构地区： 陕西省西安医学院第二附属医院消化内科,陕西西安710038

出　　处： 《海南医学院学报》 2017年第13期1737-1739,1743,共4页

摘　　要： 目的:研究乙型肝炎病毒X蛋白(HBx)对肝癌细胞株中增殖分子、侵袭分子、血管新生分子表达的影响。方法:培养肝癌细胞株HepG2并分为HBx组和对照组,分别转染pcDNA3.1-HBx质粒以及空白的pcDNA3.1质粒。转染后24h和48h,检测细胞中增殖分子Survivin、cyclinD1、c-myc以及侵袭分子CD44v6、MT1-MMP、基质金属蛋白酶(MMP)2、MMP7以及血管新生分子血管内皮细胞生长因子(VEGF)、血管生成素(Ang)-1、Ang-2、成纤维细胞生长因子(FGF-2)的mRNA表达量。结果:转染24、48h后,HBx组细胞中Survivin、cyclinD1、c-myc、CD44v6、MT1-MMP、MMP2、MMP7、VEGF、Ang-1、Ang-2、FGF-2的mRNA表达量均显著高于对照组(P<0.05)。结论:HBx能够促进肝癌细胞株中增殖分子、侵袭分子、血管新生分子的表达。 Objective：To study the effect of hepatitis B virus X protein（HBx）on the expression of proliferation molecules,invasion molecules and angiogenesis molecules in liver cancer cell lines.Methods：Liver cancer cell lines HepG2 were cultured and divided into HBx group and control group.Liver cancer cell lines HepG2 were transfected with pcDNA3.1-HBx plasmid and blank pcDNA3.1plasmid respectively.A total of 24 hand 48hafter transfection,the mRNA expression of proliferation molecules Survivin,cyclinD1 and c-myc,invasion molecules CD44v6,MT1-MMP,MMP2 and MMP7as well as angiogenesis molecules VEGF,Ang-1,Ang-2and FGF-2in cells were determined.Results：After 24 hand 48hof transfection,the Survivin,cyclinD1,c-myc,CD44v6,MT1-MMP,MMP2,MMP7,VEGF,Ang-1,Ang-2and FGF-2 mRNA expression in HBx group of cells were significantly higher than those in control group.Conclusions：HBx can promote the expression of proliferation molecules,invasion molecules and angiogenesis molecules in the liver cancer cell lines.

关 键 词： 原发性肝癌 乙型肝炎病毒 蛋白 增殖 侵袭 血管新生