作　　者： (张媛）; (谢华桃）; (朱映天）;

机构地区： 美国TissueTech生物公司研发部,佛罗里达迈阿密33173

出　　处： 《转化医学电子杂志》 2017年第8期72-75,共4页

摘　　要： 人角膜内皮细胞(HCECs)位于角膜内侧,增殖能力有限,损伤后容易失去代偿功能,发生内皮盲.目前,角膜移植是治疗该疾病的唯一有效的方法,但供体短缺等因素制约了角膜移植术的应用.因此,使用组织工程角膜作为传统角膜供体的替代物已迫在眉睫.本文回顾了近年来国内外相关研究成果,阐述了为克服接触性抑制和内皮-间质转化(EMT)的问题,学者们对HCECs的分离和体外培养方法进行改进,并总结了从形态学,关键标志物以及基因水平来鉴定HCECs的方法,为HCECs组织工程角膜内皮植片的进一步研究提供参考. Human corneal endothelial cells( HCECs) are located in the posterior cornea. They are well-known for their limited proliferative capability and therefore prone to corneal endothelial dysfunction that eventually may lead to blindness. At present,the only effective way to cure corneal endothelial dysfunction is corneal transplantation. However,because of the global shortage of donor corneas,it is vital to engineer corneal tissue in vitro that could potentially be transplanted clinically. In this review,we elaborately review recent research achivements of isolation and expansion of HCECs in order to unlock the contact inhibition and further avoid endothelial-mesenchymal transition( EMT). Also,we summarize the characterization methods from the morphology,key markers and genetics level,and provide reference for potential clinical application of corneal endothelial cell grafts.

关 键 词： 人角膜内皮细胞 分离 体外培养 鉴定