作　　者： (刘瑞）; (王志英）; (张丹丹）; (裴妙荣）;

机构地区： 山西中医学院,晋中030619

出　　处： 《药物分析杂志》 2017年第8期1509-1512,共4页

摘　　要： 目的:采用超高效液相色谱法测定羊红膻中木犀草素-7-O-β-D-葡萄糖醛酸苷和芹菜素-7-O-β-D-葡萄糖醛酸苷2种黄酮苷的含量。方法:采用BEH C_(18)色谱柱(2.1 mm×100 mm,1.7μm),以乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,流速0.4 m L·min^(-1),柱温30℃,检测波长345 nm。结果:木犀草素-7-O-β-D-葡萄糖醛酸苷质量浓度在19.38~487.6μg·m L^(-1)范围内具有良好线性关系(r=0.999 6,n=5),3批羊红膻药材中其含量分别为4.62、4.23、3.88 mg·g^(-1);芹菜素-7-O-β-D-葡萄糖醛酸苷质量浓度在21.16~423.2μg·m L^(-1)的范围内具有良好线性关系(r=0.999 7,n=5),3批羊红膻药材中其含量分别为3.81、3.36、4.01 mg·g^(-1)。结论:此方法可用于药材羊红膻中上述2个黄酮苷成分的含量测定,测定结果可为该药材质量标准的完善提供依据。 Objective： To establish an UPLC method for determination of two flavonoid glycosides luteolin-7- O-13-D-glucuronopyranoside and apigenin-7-O-13-D-glucuronopyranoside in Yanghongshan the whole plant or the radix of Pimpinella thellungiana Wolff. Methods： on a BEH Cls column（2.1 mm×50 mm, 1.7 μm）. The The chromatographic separation was performed at 30 ℃ mobile phase consisted of acetonitrile and 0. 1% formic acid water using a gradient elution. The flow rate was set at 0. 4 mL·min^-1 with the detection wavelength of 345 nm. Results ： The standard curve of the luteolin-7-O- 15 -D-glueuronopyranoside showed good linearlty in the range of 19.38 to 487.6 μg·m L^-1 （ r=0. 999 6 ）. The content of 3 batches of Yanghongshan was 4. 62 ·min^-1, 4. 23 mg·g^-1 and 3.88 mg·g^-1 for luteoli-7-O- β -D-glucuronopyranoside, respectively. The standard curve of the apigenin-7-O- 13 - D-glucuronopyranoside was linear in the range of 21.16 to 423.2 μg·m L^-1 （ r=0. 999 7 ） and the contents of 3 batches of Yanghongshan were 3.81 mg·g^-1 , 3.36 mg·g^-1 and 4. 01 mg·g^-1 for apigenin-7-O- β -D-glucuronopyranoside, respectively. Conclusion： The established method can be used for the determination of flavonoid glycosides in Yanghongshan. Moreover, the quantitative results may be useful for the quality improvement of this herbal medicine.

关 键 词： 羊红膻 黄酮苷 木犀草素葡萄糖醛酸苷 芹菜素葡萄糖醛酸苷 超高效液相色谱