作　　者： (欧阳俊峰）; (陈思扬）; (魏娜）; (张沙沙）; (鄢庆枇）; (林茂）; (马英）;

机构地区： 集美大学水产学院、农业部东海海水健康养殖重点实验室,福建厦门361021

出　　处： 《水产学报》 2017年第8期1237-1245,共9页

摘　　要： 为研究厦门近岸潮间带滩涂底泥中微型真核生物的群落结构及其对双齿围沙蚕扰动的响应,采用PCR-DGGE技术对室内模拟沙蚕生物修复过程中微型真核生物的群落结构及动态变化进行了研究。结果显示,集美滩涂底泥中微型真核生物主要分为后生动物(包括6个门:颚口动物门、环节动物门、线虫动物门、节肢动物门、软体动物门和腹毛动物门),丝足虫门,硅藻门和囊泡虫总门(又分为纤毛虫门和甲藻门)。其中囊泡虫总门的纤毛虫门为优势类群。后生动物中的线虫门为次优势类群。Peridinium quinquecorne(属于囊泡虫总门中的甲藻门)是普遍存在的优势物种。DGGE图谱聚类分析表明播种双齿围沙蚕的处理组样品中微型真核生物的群落结构聚为一支,并与未播种沙蚕的对照样品分开。研究表明,播种双齿围沙蚕对微型真核生物群落结构造成了一定的影响。沙蚕的处理组检测到了更多的线虫动物门,可能是因为双齿围沙蚕的活动促进了这些类群的生长。 The microeukaryotic community structure and response to indoor simulated bioremediation by Nereis succinea of mudflat sediments in Jimei, Xiamen, was investigated by PCR-DGGE. The results indicated that the microeukaryotes in mudflat sediments of Jimei were mainly divided into four groups： Metazoa（including 6 phyla：Gnathostomulida, Annelida, Nematoda, Arthropoda, Mollusca and Gastrotricha）, Cercozoa, Bacillariophyta and Alveolata（including Pyrrophyta and Ciliophora）. The dominant group was Ciliophora, and the subdominant group was Nematoda. Peridinium quinquecorne（belonging to Pyrrophyta） was the dominant species. Cluster analysis of DGGE profiles indicated that nereids bioturbation had influenced the microeukaryotic community, and the treatment samples with nereids clustered together and separated from the control samples without N. succinea.More Nematoda were detected in treatment samples, which probably indicated that the nereid bioturbation stimulated the growth of Nematoda.

关 键 词： 双齿围沙蚕 滩涂沉积物 微型真核生物 群落结构 生物扰动