作　　者： (李鹏飞）; (李月华）; (王春芳）;

机构地区： 山西医科大学转化医学中心,太原030001

出　　处： 《解剖学杂志》 2017年第4期408-411,共4页

摘　　要： 目的:研究干扰miR-31表达初期Notch和Hedgehog信号通路相关基因在神经干细胞(NSCs)中的表达变化。方法:利用荧光定量PCR对干扰miR-31表达初期Notch和Hedgehog信号通路相关基因在NSCs中的表达变化进行研究。结果:干扰与过表达miR-31后3 d,NSCs中的Notch信号通路相关基因Notch2的表达均增加,Jag2、Dll3和Hes1等的表达均降低;Hedgehog信号通路相关基因Wnt3的表达均增加,Bmp5与Wnt7a的表达均降低。结论:影响miR-31的表达可引发NSCs发生分化,在此过程中Notch与Hedgehog信号通路中几个基因的表达都产生相应改变,表明miR-31与NSCs分化过程相关。 Objective： To study the expression of Notch and Hedgehog signaling pathway related genes in neural stem cells after interfering with miR-31 expression in neural stem cells（NSCs）. Methods： The expression of Notch signaling pathway and Hedgehog signaling pathway related genes in NSCs was studied by fluorescence quantitative PCR. Results： Three days after interference and over-expression of miR-31, the expression of Notch signaling pathway related gene Notch2 was increased, and Jag2, Dl13 and Hes1 were decreased; the expression of Hedgehog signaling pathway related genes Wnt3 was increased, Bmp5 and Wnt7a were decreased. Conclusion： Influencing the expression of miR-31 expression can induce differentiation of NSCs. In this process, the expression of Notch and Hedgehog signaling pathway related genes is changed accordingly. These show that miR-31 is related to the differentiation of NSCs.

关 键 词： 信号通路 相关基因 神经干细胞 表达变化 干扰 信号通路 荧光定量