作 者: (胡琳琳); (韦祎); (徐玉松); (居殿春); (马光辉);
机构地区: 江苏科技大学冶金与材料工程学院,江苏张家港215600 中国科学院过程工程研究所生化工程国家重点实验室,北京100190
出 处: 《过程工程学报》 2017年第4期821-826,共6页
摘 要: 以两亲性聚合物聚乙二醇-聚(乳酸儃乙醇酸)(mPEG儃PLGA)共聚物为材料,采用复乳法结合溶剂挥发法制备mPEG儃PLGA刺突微球,用其吸附蛋白多肽类药物,考察了膜材组分比、聚乳酸/羟基乙酸(LA/GA)摩尔比、内水相与油相体积比(W1/O)、油相与外水相体积比(O/W2)、固化条件等因素对微球的形貌结构及包埋率的影响.结果表明,优化的制备条件为:mPEG/PLGA分子量比1/19,LA/GA摩尔比50/50,W1/O/0.2,O/W2/1/10,固化搅拌速率500 r/min.该条件下所制微球刺突结构特征明显,包埋率高达70%以上,比表面积是普通光滑微球的14倍. Furcella methoxy poly(ethylene glycol-L-lactic-co-glycolic acid) (rnPEG-PLGA) microspheres were prepared by double emulsion method with solvent evaporate method. The effects of polymer components, lactic acid/glycolic acid (LA/GA) molar ratio, volume ratio of internal water phase to oil phase (WJO), volume ratio of oil phase to external water phase (OAV2) and solidification rate on morphology and encapsulation of microspheres were investigated. The results showed that the microspheres with furcella morphology were prepared under the conditions of relative molecular mass ratio ofmPEG to PLGA 1/19, LA/GA molar ratio of 50/50, W1/O of 0.2/1, O/W2 of 1/10 and solidification stirring speed of 500 r/min, the encapsulation efficiency is as high as 70%, the specific surface area of furcella microspheres is as 14 times as the smooth microslaheres.