作　　者： (雒诚龙）; (赵艳坤）; (武开乐）; (邵伟）;

机构地区： 新疆农业大学动物科学学院,乌鲁木齐830052

出　　处： 《中国畜牧兽医》 2017年第8期2277-2282,共6页

摘　　要： 试验旨在研究胰岛素样生长因子-1(insulin-like growth factor 1,IGF-1)对奶牛乳腺上皮细胞(bovine mammary epithelial cells,BMECs)增殖的影响,为后期利用IGF-1调控乳腺发育奠定理论基础。以奶牛乳腺上皮细胞为材料,首先分4组分别外源添加0(对照组)、10、50、100μg/mL IGF-1且分别培养12、24、48、72h,测定抑制BMECs凋亡率的最佳浓度;然后分6组:单纯BMECs组、BMECs+IGF-1组、BMECs+LY294002组、BMECs+IGF-1+LY294002组、BMECs+RAPA组和BMECs+IGF-1+RAPA组,采用流式细胞术测定各组细胞凋亡率。结果表明,外源性添加IGF-1对BMECs凋亡率具有抑制作用,最佳浓度为50μg/mL;BMECs+IGF-1+LY294002与BMECs+IGF-1+RAPA组细胞凋亡率均极显著高于BMECs+IGF-1组(P<0.01)。推断IGF-1能够活化PI3K/Akt/mTOR信号通路,参与BMECs凋亡的调控作用,进而抑制BMECs细胞凋亡;IGF-1可能会对被抑制PI3K/Akt/mTOR信号通路产生"修复"机制,使其能够重新参与BMECs的生命进程。 The test was aimed to study the effect of insulin-like growth factor 1（IGF-1）on the proliferation of bovine mammary epithelial cells（BMECs）,and provide a theoretical basis for the subsequent regulating mammary gland development using IGF-1.The optimal IGF-1concentration for inhibiting BMECs apoptosis was obtained by measuring the apoptosis rate at 12,24,48 and 72hafter the addition of IGF-1at four groups（0,10,50 and 100μg/mL）.Then divided into six groups：BMECs group,BMECs＋IGF-1 group,BMECs＋ LY294002 group,BMECs＋IGF-1＋LY294002group,BMECs＋RAPA group and BMECs＋IGF-1＋RAPA group,and the apoptosis rates were detected by flow cytometry.The results showed that the heterologous IGF-1had an inhibitory effect on the apoptosis of BMECs with an optimal concentration of 50μg/mL.The apoptosis rates of BMECs＋IGF-1＋LY294002and BMECs＋IGF-1＋RAPA groups were extremely significantly higher than BMECs＋IGF-1group（P〈0.01）.This study suggested that IGF-1could activate the PI3K/Akt/mTOR signaling pathway and thereby inhibit the apoptosis of BMECs.Moreover,IGF-1 might also promote the ＂repair＂mechanism for the inhibited PI3K/Akt/mTOR signaling pathway,and therefore make it reparticipate in BMECs life process.

关 键 词： 胰岛素样生长因子 奶牛乳腺上皮细胞 增殖 凋亡