作　　者： (韩怡静）; (刘健）;

机构地区： 合肥工业大学生物与医学工程学院,安徽合肥230009

出　　处： 《合肥工业大学学报（自然科学版）》 2017年第8期1139-1143,共5页

摘　　要： 肥胖进程中,脂肪组织中的巨噬细胞由抗炎性的M2(2型巨噬细胞)逐渐极化成促炎性的M1(1型巨噬细胞),M1分泌的促炎性细胞直接可以导致胰岛素抵抗等肥胖相关代谢综合症。由于体外常用细菌脂多糖(Lipopolysaccharide,LPS)诱导的促炎性巨噬细胞来研究脂肪组织中巨噬细胞的极化,但其与脂肪组织中M1的表型相差甚远;另外,粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)和巨噬细胞集落刺激因子(macrophage colony stimulating factor,M-CSF)可以诱导骨髓细胞分化为骨髓来源巨噬细胞(bone marrow-derived macrophage,BMDM)。文章发现GM-CSF诱导的巨噬细胞(GM-CSF-induced macrophages,GM-BMM)高表达脂肪组织M1型标志基因CD11c与NOS2和促炎性细胞因子IL6与TNFα;与此相反,M-CSF诱导的骨髓细胞分化的巨噬细胞(M-CSF-induced macrophages,MBMM)高表达脂肪组织M2标志基因CD206和抗炎性细胞因子IL10。因此,GM-BMM和M-BMM更适合用于脂肪组织巨噬细胞极化的体外研究。 In the process of obesity, macrophages polarize from anti-inflammatory M2 to pro-inflamma-tory M1 in adipose tissues. Inflammatory cells secreted from M1 can induce insulin resistance and obe-sity related metabolic syndrome directly. Lipopolysaccharide（LPS） is often used to induce pro-inflam-matory macrophages to study macrophages polarization in adipose tissues in vitro. However, LPS-in-duced macrophages are drastically different from M1 in adipose tissues. Furthermore, granulocyte-macrophage colony stimulating factor （GM-CSF） and macrophage colony stimulating factor （M-CSF）can induce bone marrow into bone marrow-derived macrophage（BMDM）. It was found that GM-CSF-induced macrophages（GM-BMM） could strongly express adipose tissues related M1 markers genesCDllc and NOS2 and inflammatory cytokines IL6 and TNFa. On the contrary, M-CSF-induced mac-rophages（M-BMM） could highly express adipose tissues related M2 markers genes CD206 and anti-in-flammatory cytokines ILIO. Consequently, GM-BMM and M-BMM are more suitable for adipose tis-sues related macrophages polarization in vitro.

关 键 词： 粒细胞 巨噬细胞集落刺激因子 巨噬细胞集落刺激因子 巨噬细胞极化 炎性 肥胖