作　　者： (李刚）; (都林娜）; (许方程）; (王阳）; (泮琇）;

机构地区： 温州科技职业学院,浙江温州325006

出　　处： 《浙江大学学报（农业与生命科学版）》 2017年第4期493-501,共9页

摘　　要： 采用单因素实验研究温度、pH值、碳氮源、金属离子等操作参数对肠杆菌(Enterobacter sp.)菌株CV-b降解孔雀石绿的影响,并利用紫外可见光分光光度法、红外扫描光谱法和气相质谱分析法等对代谢产物进行分析。结果显示:多数供试碳源对脱色没有显著影响,而淀粉对脱色有促进效果;多数有机氮源对脱色有显著的促进效应,其中以蛋白胨的促进效果最优。在pH 5.0~10.0、温度20~50℃时,菌株CV-b对孔雀石绿的6 h脱色率在92%以上;培养6 h后,该菌株对质量浓度低于900 mg/L的孔雀石绿脱色率可达90%以上。此外,在所测金属离子中,铜离子对脱色有显著的抑制效应。酶分析数据表明,酪氨酸酶可能参与了菌株CV-b降解孔雀石绿的过程。代谢产物分析结果显示,菌株CV-b降解孔雀石绿的主要产物之一为二甲氨基二苯甲酮。总体而言,该菌株在孔雀石绿的生物修复中具有广阔的应用潜能。 Malachite green(MG)is a typical triphenylmethane dye that has been extensively used in ceramics,dyeing,textile and leather industries,etc.Meanwhile,MG can also be used as insect repellant,insecticide and anti-microbial agents in aquaculture industry.However,it has been reported that MG is difficult to degrade and has potential carcinogenicity and genotoxicity for humans,animals,and microorganisms.As a result,MG has been prohibited from being used in aquaculture by Food and Drug Administration of the United States,United Kingdom,China,European Union,and some other countries.Therefore,to remove MG residue in the aquatic environment,protect human beings and maintain the ecological balance,screening of microorganisms for biodegradation of MG is very necessary and important.Biodegradation of MG by microorganisms has gained more and more attention due to its inexpensive and eco-friendly feature.Raoultella sp.,Pandoraea sp.,Pseudomonas sp.and Arthrobacter sp.have been reported to have potential ability for biodegradation of MG.The degradation behavior was distinctly affected by culture condition and environmental factors.

关 键 词： 肠杆菌属 孔雀石绿 降解特性 酶分析 产物分析