作　　者： (东红明）; (刘媛）; (刘可可）; (邓燕）;

机构地区： 湖南工业大学绿色包装与生物纳米技术应用湖南省重点实验室,湖南株洲412007

出　　处： 《包装学报》 2017年第1期53-58,共6页

摘　　要： 以纳米金和乙酰胆碱酯酶修饰自制的纳米多孔类碳糊电极(nanoPPCPE),制备了一种新型纳米多孔乙酰胆碱酯酶(ACh E)生物电化学传感器。并根据有机磷酯类农药对AChE的特征性抑制机理,将该传感器应用于农药马拉松的检测中。实验结果表明:加入金纳米粒子做信号放大元后,传感器的峰电流大幅度增加;马拉松检测的最佳温育时间为14 min;以制备的ACh E传感器检测马拉松农药,当马拉松的质量浓度在1.0×10^(-10)~1.0×10^(-13)g/m L范围内时,酶的抑制率与其浓度的负对数值呈现出良好的线性关系,其线性方程为I=19.43C-181.4,C=-lg(ρ/(g·m L^(-1))),线性相关系数R=0.996,最低检测限为5.0×10^(-14)g/m L。 A new nano porous acetylcholinesterase(AChE)bioelectrochemical sensor was constructed based ongold nanoparticles(GNPs)and AChE modified self-made nano porous pseudo carbon paste electrodes(nano-PPCPE).The sensor was applied in the detection of marathon based on the representative inhibition mechanism betweenorganophosphorus pesticide and AChE.The experimental results showed that the peak current of the sensor dramaticallyincreased after adding gold nanoparticleas signal amplification.The best incubation time for marathon test was14min.The prepared AChE sensor was used to detect marathon.When the concentration of marathon was within the rangefrom1.0×10-10to1.0×10-13g/mL,there was a good linear relationship between enzyme inhibition and the negativelogarithmic of enzyme's concentration with the linear equation being I=19.43C-181.4,C=-lg(ρ/(g·mL-1)),the linearcorrelation coefficient being R=0.996,and the detection limit of5.0×10-14g/mL.

关 键 词： 纳米金 乙酰胆碱酯酶 生物传感器 马拉松 检测限