作　　者： (吴中俭）; (屈海龙）;

机构地区： 首都医科大学宣武医院血管外科,北京100053

出　　处： 《海南医学院学报》 2017年第11期1456-1458,1462,共4页

摘　　要： 目的:研究血管紧张素1-7(Ang1-7)对氧化应激所致内皮细胞损伤的影响。方法:培养人脐静脉内皮细胞(HUVECs)并分为空白对照组、过氧化氢组和不同剂量Ang1-7组(1μmol/L、2μmol/L、4μmol/L Ang1-7)组。检测细胞的增殖活力、细胞培养基中抗氧化酶的含量、细胞中内质网应激分子的含量。结果:处理6、12、18、24h后,过氧化氢组细胞的CCK-8增殖活力值显著低于空白对照组,1μmol/L、2μmol/L、4μmol/L Ang1-7组细胞的CCK-8增殖活力值显著高于过氧化氢组且Ang1-7剂量越大,细胞的CCK-8增殖活力值越高;处理24h后,过氧化氢组细胞培养基中SOD、GSH-Px、HO-1、CAT的含量显著低于空白对照组,细胞中GRP78、XBP1、CHOP的含量显著高于空白对照组;1μmol/L、2μmol/L、4μmol/L Ang1-7组细胞培养基中SOD、GSH-Px、HO-1、CAT的含量显著高于过氧化氢组,细胞中GRP78、XBP1、CHOP的含量均显著低于过氧化氢组,且Ang1-7剂量越大,培养基及细胞中上述分子的变化越显著。结论:血管紧张素1-7对氧化应激所致内皮细胞损伤具有保护作用。 Objective：To study the effects of angiotensin 1-7（Ang1-7）on endothelial cell injury caused by oxidative stress.Methods：Human umbilical vein endothelial cells（HUVECs）were cultured and divided into blank control group,hydrogen peroxide and different Ang1-7dose groups（1μmol/L,2μmol/L and 4μmol/L Ang1-7groups）.The cell proliferation activity,the contents of antioxidant enzymes in cell culture medium,and the contents of endoplasmic reticulum stress molecules in cells were determined.Results：After 6,12,18 and 24hof treatment,CCK-8proliferation activity of hydrogen peroxide group were significantly lower than those of blank control group,CCK-8proliferation activity of 1μmol/L,2μmol/L and 4μmol/L Ang1-7groups were significantly higher than those of hydrogen peroxide,and the larger the Ang1-7dose,the higher the CCK-8proliferation activity;after 24 hours of treatment,SOD,GSH-Px,HO-1and CAT contents in cell culture medium of hydrogen peroxide group were significantly lower than those of control group,as well as GRP78,XBP1 and CHOP contents in cells were significantly higher than those of control group;SOD,GSH-Px,HO-1and CAT contents in cell culture medium of 1μmol/L,2μmol/L and 4μmol/L Ang1-7groups were significantly higher than those of hydrogen peroxide group,as well as GRP78,XBP1 and CHOP contents in cells were significantly lower than those of hydrogen peroxide group,and the larger the Ang1-7dose,the more significant the changes of above molecules in cell culture medium and cells.Conclusions：Angiotensin 1-7 has protective effect on the endothelial cell injury caused by oxidative stress.

关 键 词： 血管紧张素 氧化应激 内皮细胞 内质网应激