作　　者： (杨秀乔）; (苗琳）; (谢洪良）; (黄磊）; (苏世达）; (潘顺勇）; (曾发明）; (翟滇）; (程宝文）;

机构地区： 昆明医科大学法医学院,云南昆明650500

出　　处： 《中国法医学杂志》 2017年第4期385-387,392,共4页

摘　　要： 目的建立快速、准确检测制式长枪上基因分型的新方法。方法联合使用直扩法、硅膜法对48支制式长枪上5个不同部位共240份检材进行DNA检测。结果联合使用直扩法及硅膜法,在48支制式长枪中检测出DNA完整分型42支,检出率达87.50%。结论联合使用直扩法、硅膜法,可有效快速、准确地检测出制式长枪上DNA多态性。 Objective A new methodology was established to efficiently obtain the genotype of DNA remained on standard long gun. Methods Direct PCR and silicon membrane method were combined to detect DNA polymorphism of a total of 240 samples at 5 different positions from 48 standard long guns. Results Combining direct PCR and silicon membrane method, we obtained full DNA profiles in 42 out of 48 standard long guns, with a detection rate up to 87.50%. Conclusion The results demonstrate that the combination of direct PCR and silicon membrane method provide a quick and accurate way to detect DNA polymorphism on the standard long gun.

关 键 词： 法医物证学 直扩法 硅膜法 制式长枪 基因分型