作　　者： (郝晓明）; (孙进广）; (苟春宝）; (樊哲仁）; (刘安丽）; (赵武军）; (张郑）;

机构地区： 重庆市巴南区公安分局刑事科学技术室,重庆401320

出　　处： 《中国法医学杂志》 2017年第4期379-381,共3页

摘　　要： 目的采用磁珠直接吸附法对人体尿液、唾液、血液3种体态生物检材中的游离DNA进行提取检验,为法医物证中游离DNA的研究及检验工作提供参考。方法对3种生物检材采取离心吸取上清液的方法分离游离DNA,然后采用磁珠直接吸附法进行提取纯化,Identifiler-Plus试剂盒进行复合扩增后常规STR检测。结果在3种检材中均检出了游离DNA,其中血液中游离DNA检出率为100%,唾液为90%,尿液为70%。结论人体体态生物检材中存在游离DNA,同时磁珠直接吸附法可高效、快捷的提取生物检材中的游离DNA。 Objective The magnetic beads direct adsorption method was used to extract the cell-free DNA（cf DNA） from three kinds of human humoral biological samples, including urine, saliva and blood, as to provide a reference for forensic cf DNA research and forensic inspection. Methods The cf DNA was isolated from humoral samples by centrifuging, and the cf DNA was extracted with the method of magnetic beads direct adsorption. Then the samples were sequentially amplified with Identifiler-Plus amplication kit, and the STR genotyping was detected by ABI 3500 Analyzer. Results The cf DNA was detected from all the three kinds of samples. The detection rate of cf DNA from the blood samples was 100%, the saliva was 90%, and the urine was 70%. Conclusion The results suggest that human humoral biological samples contain cf DNA. What＇s more, the magnetic beads direct adsorption method can be used to extract cf DNA efficiently and conveniently.

关 键 词： 法医物证学 游离 磁珠 直接吸附法 生物检材