作　　者： (刘悦）; (王晓雨）; (曲春安）; (胡飞）; (胡亚暖）; (唐胜建）;

机构地区： 潍坊医学院,山东潍坊261041

出　　处： 《山东医药》 2017年第29期8-11,共4页

摘　　要： 目的探讨miRNA-520e对人宫颈癌HeLa细胞FOXL2基因的调控作用及对细胞迁移、增殖的影响。方法应用生物信息学分析软件(Target Scan Human 7.1)预测miRNA-520e可能结合于FOXL2 mRNA 3'UTR端,以HeLa细胞作为试验研究对象,将细胞随机分为阴性对照组(加入无关序列NC Fam mimics)、正常对照组(不加任何干预)、miRNA-520e组(加入miRNA-520e mimics)。运用脂质体法通过Lipofectamine 3000 Reagent将各miRNA mimics转染各组HeLa细胞,48 h后通过实时荧光定量PCR及Western blotting法检测FOXL2 mRNA及蛋白表达;转染24 h后采用细胞划痕试验检测细胞迁移能力,使用ACEA xCELLigence RTCA DP细胞分析仪检测细胞增殖能力。结果 miRNA-520e组HeLa细胞FOXL2 mRNA和蛋白的表达量较正常对照组及阴性对照组降低,且miRNA-520e组细胞的迁移和增殖能力较正常对照组及阴性对照组增强(P均<0.05)。结论 miRNA-520e可抑制HeLa细胞FOXL2 mRNA和蛋白的表达,促进细胞迁移和增殖。 Objective To investigate the regulative effects of microRNA-520 e on FOXL2 gene of HeLa cells and its influence on the migration and proliferation of HeLa cells. Methods Using bioinformatics（ Target Scan Human 7. 1） to predict that miRNA-520 e may bind to FOXL2 mRNA 3 ＇UTR. HeLa cells were randomly divided into the negative control group（ added with NC Fam mimics）,control group（ no treatment）,and miRNA-520 e group（ added with miRNA-520 e mimics）. We transfected miRNA mimics into HeLa cells in each group by using Lipofectamine 3000 Reagent. At 48 h after transfection,the FOXL2 mRNA and protein expression was detected by real-time fluorescent quantitative PCR and Western blotting,respectively. Besides,at 24 h,we used Scratch test to detect the cell migration ability,and the ACEA xCELLigence RTCA DP cell analyzer to detect the cell proliferation. Results The expression of FOXL2 mRNA and protein in the miRNA-520 e group was lower than that in the control group and negative control group,and the migration and proliferation abilities of the miRNA-520 e group were stronger than those of the control group and negative control group（ all P 0. 05）.Conclusion miRNA-520 e could inhibit the expression of FOXL2 mRNA and protein in HeLa cells,and promote the migration and proliferation of HeLa cells by targeting FOXL2.

关 键 词： 宫颈癌 细胞 微小 基因 细胞迁移 细胞增殖