作　　者： (姚琴）; (胡广斌）; (邢玉平）; (朱兆香）; (郭树林）; (陈孝仁）;

机构地区： 江苏省农垦农业发展股份有限公司谅港分公司,江苏东台224200

出　　处： 《大麦与谷类科学》 2017年第4期40-44,共5页

摘　　要： 近年来,江苏沿海地区小麦普遍出现基部叶鞘腐烂、叶枯等症状,造成一定的产量损失。为明确病原菌,对病株进行组织分离得到1株真菌。根据形态学特征,初步鉴定为微结节霉属真菌(Microdochium nivale)。利用PCR技术对病原菌的核糖体间隔转录区域(ITS)序列进行扩增,测序比对发现与M.nivale的同源相似性为99%。经致病性测定,确定M.nivale为引起小麦基部叶鞘腐烂、叶枯等症状的病原菌,结合先前报道称之为雪腐叶枯病。 In recent years, wheat grown in Jiangsu coastal areas developed disease symptoms manifested by decaying leaf sheaths and withered leaves, resulting in a certain degree of yield loss. A fungal isolate was obtained from diseased wheat tissues. Based on its morphological features, the fungal isolate was initially identified as Microdochium nivale. Its internal transcribed space（ITS） was amplified through PCR and subsequently sequenced. The comparison between its ITS sequence and that of M. nivale revealed that they have 99% homology. Furthermore, the isolated M. nivale demonstrated the ability to cause wheat sheath rot and leaf blight in the assay of pathogenicity determination. Taken together, the above results confirm that the pathogenic fungus causing root and crown rot of wheat is M. nivale. Based on the results in this study as well as previous reports, the disease caused by this pathogen is found to be snow mold disease.

关 键 词： 小麦 鞘腐 雪腐叶枯病 致病性