作　　者： (谢振华）; (田继微）;

机构地区： 清华大学深圳研究生院健康科学和技术重点实验室,广东深圳518055

出　　处： 《大麦与谷类科学》 2017年第4期10-15,共6页

摘　　要： 利用Excel具有的数据管理功能实现粳稻全蛋白质组所有蛋白序列有序化管理,进而实现对粳稻全蛋白质组蛋白序列排序和归类。本研究将粳稻全蛋白质组48 905条蛋白序列的24个理化性质参数、蛋白名称、蛋白登录号码和蛋白序列组成数据矩阵,导入Excel中。根据蛋白不同理化性质参数进行排序,筛选得到特定理化性质的蛋白;根据蛋白名称排序,实现蛋白质家族成员和蛋白选择剪切变异体的系统归类排序;通过对粳稻全蛋白质组理化参数分布的可视化,促进对粳稻全蛋白质组更直观和全面的认识。 Owing to its function of data management, Excel can be used to sort and cluster all protein sequences of the complete proteome of japonica rice. In this study, a data matrix was constructed, comprising 24 physicochemical parameters, names, accession numbers, and sequences of 48 905 proteins in the complete proteome of japonica rice; and this data matrix has been imported into an Excel table for orderly management, clustering, and querying. Any proteins with some particular physicochemical features can be screened out from the complete proteome of japonica rice by orderly management; all members of a protein family or protein splice variants can be systematically clustered by alphabetically sorting the name column. Such an Excel table provides an overview of the complete proteome of japonica rice by visualizing the distribution of the physicochemical parameters of all proteins. Therefore, this study creates a tool that is instrumental in comprehensive and in-depth understanding of the complete proteome of Japonica rice.

关 键 词： 粳稻 全蛋白质组 蛋白 氨基酸 等电点 疏水性