作　　者： (韩雨薇）; (刘秀娟）; (高阳）; (李晓明）;

机构地区： 沈阳军区总医院神经外科,沈阳110016

出　　处： 《实用药物与临床》 2017年第8期863-867,共5页

摘　　要： 目的探讨齐墩果酸对蛛网膜下腔出血后早期脑损伤的保护作用,阐释其可能的机制。方法 54只成年雄性SD大鼠分成3组(假手术组、SAH模型组、齐墩果酸给药组),每组18只。采用颈内动脉穿刺法建立大鼠SAH模型,造模1 h后给药,24 h后进行神经功能损伤评分;检测脑水含量及脑伊文思蓝浸出;并利用Western blot方法检测脑组织Src、VEGF、ZO-1、Claudin-5、Occludin蛋白的表达量。结果齐墩果酸高剂量给药可有效改善神经功能损伤(P<0.01);降低脑组织含水量和脑血管通透性(P<0.01);增加ZO-1、Claudin-5、Occludin蛋白的表达,降低VEGF水平,抑制Src激活(P<0.05)。结论齐墩果酸对SAH后早期脑损伤有显著的保护作用,可改善脑水肿,其作用机制可能与增加脑组织紧密连接蛋白的表达有关。 Objective To explore the effect of oleanolic acid（OA） on early brain injury following subarachnoid hemorrhage（SAH） and explain its possible mechanism.Methods Totally 54 male SD rats were divided into 3groups：sham group（n = 18）,SAH group（n = 18） and OA-treated SAH group（n = 18）.The endovascular puncture model was used to induce SAH.Neurological score,brain water content and blood-brain barrier（BBB） were evaluated at 24 h after SAH.The protein expression of ZO-1,VEGF and Src was determined by Western blot.Results Highdose OA administration could significantly improve the neurological scores（P〈0.01）,reduce the brain water content and BBB permeability（P〈0.01）,up-regulate the expression of ZO-1,Claudin-5 and Occludin,and inhibit the expression of VEGF and Src phosphorylation（P〈0.01）.Conclusion OA can protect against the early brain injury after SAH and improve brain edema by increasing the expression of tight junctions.

关 键 词： 齐墩果酸 蛛网膜下腔出血 脑水肿 紧密连接