作　　者： (张霄龙）; (任杰）; (王正荣）; (应正祺）; (王霞）; (黄禹瑶）; (后望）; (成姝婷）;

机构地区： 四川大学华西基础医学与法医学院,四川成都610041

出　　处： 《四川生理科学杂志》 2017年第3期112-114,共3页

摘　　要： 目的:研究成都地区污染空气中PM2.5微粒对大鼠学习记忆能力的影响。方法:Morris水迷宫筛选后的雄性SD大鼠32只,随机分为:对照组、PM2.5低剂量组、PM2.5中剂量组、PM2.5高剂量组(n=8)。用EM-2062A颗粒物采样器采集2016年7月14日至7月30日成都市区污染严重区域空气中的PM2.5微粒,制成混悬液后对大鼠进行气管滴注染毒处理,低、中、高剂量组PM2.5混悬液分别为0.96mg·kg^(-1)、3.84mg·kg^(-1)和15.36mg·kg^(-1),3周后以水迷宫实验对比其学习记忆能力,ELISA检测其肺组织IL^(-1)β的水平。结果:与对照组相比,染毒大鼠的逃避潜伏期均有所增加,且高剂量组增加最多(P<0.05),而有效区域运动时间长度则与染毒剂量呈反比。结论:成都地区污染空气中PM2.5微粒能够损害大鼠的学习记忆能力,这种损害与PM2.5微粒的浓度有关。 Objective：To investigate the effect of PM2.5 in the air of Chengdu on rats′spatial learning and memory ability by Morris water maze.Methods：Thirty-two male SD rats screened by Morris water maze were randomly divided into control group,PM2.5 lower dosage group,PM2.5 middle dosage group,and PM2.5 higher dosage group（n=8）.The rats were exposed to PM2.5 ,which was collected by EM-2062 AParticulates Sampler from July 14^（th） to 30^（th） of 2017 in Chengdu,by intratracheal instillation,and the exposing dosage was depended on rats′mass,that is 0.96 mg·kg^（-1） in lower dosage group,3.84 mg·kg^（-1） in middle dosage group,and 15.36 mg·kg^（-1） in higher dosage group.Learning abilities of those rats were estimated with Morris water maze 3weeks later,and the IL^（-1）β level was tested by ELISA.Results：Compared to the control,the escape latency time of treated rats was longer,and the higher dosage group has the longest escape latency time（P〈0.05）,while there was a reverse correlation between the effective regional time of rats and PM2.5 dosage.Conclusion：PM2.5 in the polluted air of Chengdu has the ability to impair rats′ability of learning and memorizing,and this impairment was associated to the concentration of PM2.5 .

关 键 词： 成都 大鼠 学习记忆能力